CEA重组痘苗病毒免疫治疗CEA阳性肿瘤机制的研究  

A study of the mechanism of the effect of a recombinant vaccinia virus expressing human cartcinoembryonic antigen on CEA positive tumor

作　　者：赵青[1] 陶莎[2] 杨洁[2] 罗超权[2] 

机构地区：[1]广州医学院病生教研室工作,广州510182 [2]中山医科大学生物化学教研室,广州510089

出　　处：《中国免疫学杂志》2001年第2期79-81,共3页Chinese Journal of Immunology

基　　金：广州市科委基金!资助项目 (JB0 2 98 5 0 18 0 1)

摘　　要：目的 :探讨癌胚抗原重组痘苗病毒 (rV CEA)治疗癌胚抗原 (CEA)阳性肿瘤的机制。方法 :使用我室构建的rV CEA腹腔接种供体C5 7 BL小鼠后取其脾细胞过继转移给经6 0 Coγ射线亚致死量照射的荷CEA+ HePa肝癌细胞的C5 7 BL小鼠 ,并同时检测该供体小鼠脾细胞体外杀瘤细胞效应。结果 :接种了rV CEA的供体小鼠的脾细胞过继免疫给受体 ,具有明显抑制CEA阳性肿瘤细胞生长的作用。结论 :本研究提示rV CEA对CEA阳性肿瘤的抑制作用可能主要是通过CEA特异的免疫反应激活T细胞而实现的 ,其机制值得进一步研究。Objective:To explore antitumor mechanism of a recombinant vaccinia virus expressing human carcinomembryonic antigen(rV CEA).Methods:C57bl/6 mice were immunized three times with rV CEA.Six weeks later,the splenocytes from rV CEA immunized donors were adoptively tranfered in sublethally irradiated CEA + HePa bearing recipients,meanwhile the antiumor effect of the donor splencytes in virtro was detected.Results:Splenocytes from rV CEA immunized donors expressed strong antiumor activity in CEA positive tumorbearing recipients,whereas W VV immunized and nonimmune donor cell did not.The antiumor activity was abolished when immune T cells were depleted before splencytes transfer.Conclusion:It was demonstrated that antiumor acivity induced by rV CEA was essentially mediated by immune T cells which were activated by CEA specific immune response.Adoptive immunotherapy strategies may have implications for both the study of rV CEA and the development of potential clinical applications for tumor immunotherapy.

关 键 词：癌胚抗原 重组痘苗病毒 免疫治疗 CEA阳性肿瘤 

分 类 号：R730.51[医药卫生—肿瘤]