外源基因转染细胞技术的研究进展  

Advances in Technology of Heterologous Genes Transfecting Cells

作　　者：王月丽[1] 魏继楼[1] 程红蕾[1] 刘芳[1] 罗素梅[1] 王若雨[1] 

机构地区：[1]大连大学附属中山医院肿瘤科,辽宁大连116001

出　　处：《现代生物医学进展》2014年第7期1382-1384,1270,共4页Progress in Modern Biomedicine

基　　金：国家自然科学基金项目(30970869)

摘　　要：细胞转染技术是分析细胞内基因及基因产物功能的重要工具。它不仅是基因功能研究、基因免疫的理论和技术基础,也是研究基因表达调控、突变分析等的常规工具。同时也是体内应用的重要过程,比如基因治疗、疫苗接种、药物开发等。在过去的40年里,已开发了多种外源基因转染细胞的方法。主要包括以病毒介导的外源基因转染细胞方法和非病毒介导的细胞转染方法。其具体可细分为三类:即生物学方法、化学方法、物理方法。这些方法的提出使外源分子如DNA、RNA等导入特定细胞并表达目的基因及产生特定功能的蛋白质分子得以实现。理想的细胞转染方法应该具有较好的生物降解性、稳定性、靶向性强、有助于基因的表达并能应用于基因方面疾病的治疗。但每种方法都有自己的优势和不足,应根据具体实验的设计和目的来选择最佳细胞转染方法.Transfection is a powerful analytical tool enabling study of the function of genes and gene products in cells. It is the theoretical and technical basis of the study of gene function,genetic immunization theory; it is also conventional tools of gene expression and regulation, mutation analysis; and even is a critical process for in vivo applications such as gene therapy, vaccination, and drug development. Over the last 40 years, multiple different methods of nucleic acid delivery have been developed. These include viral methods and non-viral methods, which can be fi.u＇ther subdivided into three groups：biological, chemical, and physical. These methods have advanced to make it possible to deliver foreign nucleic acids （DNAs and RNAs） into the special cells and express the aim gene and protein. An ideal gene delivery system should be biodegradable, nontoxic, stable, and target-specific, should assist to improve gene expression, and be able to be used to treat genetic diseases.However, each method has its own advantages and disadvantages so the optimum method depends on experimental design and objective.

关 键 词：细胞 转染技术 基因 

分 类 号：R-331[医药卫生]