液相色谱-质谱检测3种不同属新鲜黄芩中8种成分  

Determination of 8 Components in Fresh Samples of 3 Scutellaria Species Using On-Line Liquid Chromatography-Tandem Mass Spectrometry

作　　者：董喆[1] 黄湘鹭[1] 曹进[1] 张庆生[1] 肖盛元[2] Ian B.Cole Susan J.Murch 

机构地区：[1]中国食品药品检定研究院食品化妆品检定所,北京100050 [2]北京理工大学生命科学院,北京100081 [3]卑诗大学奥根分校科学与艺术学院化学系

出　　处：《食品科学》2014年第4期131-136,共6页Food Science

基　　金：科技部国际合作课题(2011DFA31440);"十二五"国家科技支撑计划项目(2012BAK08B02)

摘　　要：目的:利用在线的方式,建立对3个属的新鲜黄芩样品中8种成分(褪黑素、5-羟色胺、吲哚-3-乙酸、金丝桃素、黄芩苷、黄芩素、汉黄芩苷、野黄芩苷)的液相色谱-质谱联用测定方法。方法:植物样品的匀浆与适量填料混合后,被填入相应的样品仓,通过杂质去除,目标物质富集洗脱等步骤,用于成分测定。结果:测定黄芩样品中8种成分,方法的检出限及定量限条件的回收率均大于90%,重复性及稳定性相对标准偏差不大于3.1%。结论:可以用于对黄芩原料中功效成分的评价。Objective： An on-line liquid chromatography-tandem mass spectrometry method was developed to determine 8 components in fresh Scutellaria samples from three germplasm sources. Methods： Mixtures of homogenated tissue samples and appropriate amounts of packing material were packed in sample chamber. After removing matrix impurities and enriching the target analytes, 8 components in fresh Scutellaria samples were determined. Results： The recoveries were greater than 90% at the limits of detection and quantification. Meanwhile, the RSDs of repeatability and stability were less than 3.1%. Conclusion： The method developed can be used for the determination of active components in Scutellaria samples.

关 键 词：液相色谱-质谱法 中国黄芩 北美黄芩 南美黄芩 

分 类 号：O657.63[理学—分析化学]