湿法消解-原子荧光光谱法测定湘葛一号中的砷、汞、铅  

Determination of Arsenic, Mercury and Lead in Radix Puerariae by Atomic Florescence Spectrophotometry with Wet Digestion

作　　者：叶惠煊[1] 谭舟 刘向前[1] 周斌文 熊力夫 

机构地区：[1]湖南中医药大学药学院,湖南省中药现代化研究重点实验室,湖南长沙410208 [2]长沙市雨花区日成晟食品添加剂厂,湖南长沙410014 [3]长沙市葛根工程技术研究中心,湖南长沙410000

出　　处：《食品科学》2014年第4期151-154,共4页Food Science

基　　金：湖南省自然科学基金重点项目(11JJ2042);长沙市科技局科技计划重点项目(K1207010-21);湖南中医药大学药物分析学"十二五"校级重点学科建设项目

摘　　要：3批不同采收期湘葛一号样品经硝酸．高氯酸的混合液湿法消解后，用原子荧光光谱法测定As、Hg和Pb的含量。As、Hg和Pb的线性范围分别为0～16．0、0～5．0ng／mL和0-40．0ng／mL；相关系数r分别为0．9999、0．9990和0．9997；检出限分别为0．050、0．012μg／L和0．038μg/L（n=11）；样品平均回收率分别为98．73％、101．22％和99．43％（n=6）。该法方法简便、准确、精密度好，可用于湘葛一号中As、Hg和Pb的含量的快速测定。A method for determination of the contents of heavy metals including As, Hg and Pb in 3 batches of Radix Puerariae （Pueraria thomsonii Benth cultivar ＂Xiangge No. 1＂ grown in Hunan province harvested at different times） was developed using atomic fluorescence spectrophotometry in combination with wet digestion. Samples were wet digested by nitric acid and perchloric acid and then detected by hydride generation atomic florescence spectrophotometry. The linear ranges for As, Hg and Pb were 0-- 16.0, 0--5.0 and 0--40.0 ng/mL, respectively, with correlation coefficients of 0.999 9, 0.999 0 and 0.999 7, respectively. Their limits of detection were 0.050, 0.012 and 0.038μg/L （n = 11）, respectively. Their recovery rates were 98.73%, 101.22% and 99.43% （n = 6）, respectively. This method allows simple, fast and accurate determination of As, Hg and Pb in Radix Puerariae.

关 键 词：湿法消解 原子荧光光谱法 湘葛一号 砷 汞 铅 

分 类 号：R284.1[医药卫生—中药学]