白细胞介素-24联合顺铂对宫颈癌裸鼠移植瘤抑制及化疗增敏作用研究  

Inhibitory effect and chemosensitization of IL-24 in combination with cisplatin in nude mice transplanted with human cervical cancer

作　　者：马才媛 李莉[2] 

机构地区：[1]山西医科大学研究生学院,太原030001 [2]山西医科大学第一医院妇产科

出　　处：《中国药物与临床》2014年第3期280-283,共4页Chinese Remedies & Clinics

基　　金：山西省自然科学基金(2007011121)

摘　　要：目的探讨基因重组质粒pDC316-hIL-24、顺铂(DDP)单独及联合应用对人宫颈癌裸鼠移植瘤抑制及化疗增敏作用。方法将人宫颈癌Siha细胞株接种BALB/c雌性裸鼠,建立动物模型。随机将造模成功的裸鼠分为:PBS组、空质粒组、白细胞介素(IL)-24组、半量顺铂组、全量顺铂组、IL-24+半量顺铂组、IL-24+全量顺铂组共7组。观察裸鼠一般状况及肿瘤生长情况,实验结束后采用聚合酶链反应(PCR)法测定移植瘤中IL-24mRNA的表达,免疫组织化学染色法观察移植瘤增殖细胞核抗原(PCNA)的变化。结果①IL-24基因成功转染及表达。②IL-24+全量顺铂组、IL-24+半量顺铂组、IL-24组、全量顺铂组抑瘤率分别为74.0%、63.9%、33.9%、36.3%高于PBS、空质粒、半量顺铂组(P<0.01),其中IL-24+全量顺铂组、IL-24+半量顺铂组抑瘤率明显优于其他组,且IL-24+半量顺铂组其生命活动和体重无明显改变,后3组抑瘤率差异无统计学意义(P>0.05)。③PBS组、空质粒组、IL-24组、半量顺铂组、全量顺铂组、IL-24+半量顺铂组、IL-24+全量顺铂组裸鼠肿瘤组织中PCNA指数分别为0.774±0.087、0.749±0.064、0.589±0.017、0.731±0.076、0.641±0.101、0.462±0.039、0.400±0.091(P<0.01)。结论 IL-24、顺铂联合应用可提高抗宫颈癌裸鼠移植瘤生长作用,其作用可能与协同抑制了肿瘤组织内PCNA的表达有关。Objective- To investigate the inhibitory effect and chemosensitization of pDC316-hIL-24, a gene re- combinant plasmid, alone or in combination with cisplatin on human cervical cancer in nude mice. Methods The human Siha cells were inoculated subcutaneously into the hind flank region of female BALB/c mice to establish xenograft models which were randomly allocated to PBS group, empty plasmid group, IL-24 group, half-dose DDP group, full-dose DDP group, IL-24＋half-dose DDP group and IL-24＋full-dose DDP group. The general conditions of nude mice and tumor growth were observed. The expression of IL-24 in transplanted tumors was identified by poly- merase chain reaction. Immunohistochemical staining was employed to detect the proliferating cell nuclear antigen （PCNA） in transplanted tumors. Results IL-24 was successfully transfected into and expressed in nude mice xenograft models. The tumor inhibitory rates in the IL-24＋full-dose DDP group, IL-24＋half-dose DDP group, IL-24 and full-dose DDP group （74.0%, 63.9%, 33.9% and 36.3%） were significantly higher than the remaining groups （all P〈0.01）. The IL-24＋full-dose DDP group and IL-24＋half-dose DDP group yielded a higher tumor inhibitory rate （both P〈0.01）, while the latter group did not alter the vital signs or weight compared with all other groups. The difference in tumor inhibitory rate was not statistically significant among the latter three groups （P〉0.05）. The PCNA of tumor tissue was 0.774±0.087, 0.749±0.064, 0.589±0.017, 0.731±0.076, 0.641±0.101, 0.462±0.039 and 0.400_＋0.091, respectively （all P〈0.01）. Conclusion The enhanced tumor inhibitory effects conferred by combined intervention with IL-24 and DDP might be associated with inhibition of PCNA expression.

关 键 词：宫颈肿瘤 白细胞介素类 顺铂 增殖细胞核抗原 

分 类 号：R737.33[医药卫生—肿瘤]