MIF基因修饰的瘤苗免疫小鼠后T细胞数量和功能的分析  

The number and function analysis of T cell from mice immunized with MIF gene modified tumor vaccine

作　　者：刘艳君[1] 田野苹[1] 曹雪涛[1] 张明徽[1] 厉永建[1] 周正芳[1] 郑玲莉[1] 

机构地区：[1]第二军医大学基础医学部免疫学教研室,上海200433

出　　处：《第二军医大学学报》2001年第1期32-35,共4页Academic Journal of Second Military Medical University

基　　金：国家自然科学基金资助项目! (396 70 2 80 )

摘　　要：目的 :研究 T细胞在巨噬细胞游走抑制因子 (MIF)基因修饰的瘤苗诱导抗肿瘤免疫反应中的作用。 方法 :将重组MIF腺病毒转染小鼠 FBL 3红白血病细胞制成的瘤苗接种于小鼠体内 ,观察脾脏和淋巴结中 T细胞数量和功能的改变。 结果 :经 MIF基因修饰的瘤苗免疫后 ,小鼠脾细胞和腹股沟引流淋巴结细胞数量明显增多 ,脾细胞 CTL活性显著增强 ,引流淋巴结中 CD3+ 、CD2 8+ 、CD4+ 和 CD8+ T细胞百分率均较对照组增加。MIF基因修饰的瘤苗免疫对小鼠受野生型 FBL 3肿瘤细胞再攻击具有明显的保护作用。结论 :MIF基因修饰的瘤苗能诱导 T细胞介导的抗肿瘤免疫反应 。Objective: To investigate the role of T cell in the antitumor immune responses induced by MIF gene modified tumor vaccine. Methods: MIF gene was transferred into FBL3 erythroleukemia cell by adenovirus carrier and a new type of tumor vaccine was prepared. The changes of the number and the function of T cell in spleen and lymph node was observed. Results: After the mice were immunized with MIF gene modified FBL3 vaccine, the number of lymphocyte in spleens and lymph nodes increased markedly and the specific CTL activities of splenocytes also increased greatly. FACS analysis showed that the CD3 +, CD4 +, CD8 + T cells and CD28 positive cells in draining lymph nodes of MIF FBL3 group mice increased more markedly than that of control groups. When the wild type FBL3 cells were injected into the mice immunized with MIF gene modified FBL3 vaccine, the growth of tumors were obviously inhibited and the survival rate of the mice was increased. Conclusion: It is suggested that MIF gene modified tumor vaccine can induce specific antitumor immune responses mediated by T cells and may be a candidate for gene therapy of tumor.

关 键 词：巨噬细胞游走抑制因子 疫苗 T淋巴细胞 基因修饰 肿瘤 基因治疗 

分 类 号：R730.5[医药卫生—肿瘤] R392.11[医药卫生—临床医学]