一株分离自新疆细虫草菌核的弯颈霉鉴定及抑菌研究  

Identification and antibacterial effect research of a Tolypocladium strain isolated from sclerotium of Ophiocordyceps gracilis in Xinjiang

作　　者：索菲娅[1] 黄罗冬[2] 虞泓[2] 

机构地区：[1]新疆大学生命科学与技术学院,新疆乌鲁木齐830046 [2]云南大学中草药生物资源研究所云百草实验室,云南昆明650091

出　　处：《中国中药杂志》2014年第6期965-971,共7页China Journal of Chinese Materia Medica

基　　金：国家自然科学基金项目(31160073);新疆教育厅高等学校科研计划项目(XJEDU2010I12);新疆维吾尔科技支疆项目(201091245)

摘　　要：目的:开发利用新疆细虫草资源,为新疆细虫草相关真菌的研究和开发利用提供基础资料。方法:研究从新疆细虫草菌核中分离得到一种白色菌株,编号为SFYT002,采用形态学观察和ITS以及18SrDNA测序,在GenBank比对,进行系统进化树分析,并采用滤纸片法研究其体外抗菌作用。结果:菌株SFYT002主要的特征为菌落白色棉绒状,瓶梗长在分枝的分生孢子梗上,瓶梗基部膨大,顶部急剧变细,部分颈部弯曲,孢子小且透亮,孢子有出芽现象,常形成聚集孢子头,分生孢子球形,亚球形至椭圆形。ITS和18SrDNA序列分析表明,弯颈霉属为单系发生,菌株SFYT002与膨大弯颈霉聚为一支。研究其抑菌作用表明,菌株SFYT002发酵液及其不同极性的提取物对4种常见致病菌,均有很强的抑制作用。结论:通过形态学和分子生物学特征将菌株SFYT002鉴定为膨大弯颈霉,具有较强的抑菌活性。Objective： To promote development and utilization of Ophiocordyceps gracilis in xinjiang and provide basic data for researching and sustainable developing medicine fungus related to O. gracilis. Method： A white strain SFYT002 isolated from the sclerotium of O. gracilis in Xinjiang was researched by morphological observation, ITS and 18SrDNA sequencing. The ITS and 18SrDNA sequences of the strain were determined, BLAST was compared with the other sequences of Tolypocladium in GenBank. The phylogenetic trees of ITS and 18SrDNA sequences were analyzed in Tolypocladium. In addition, the filter paper method was used to study the antibacterial effects. Result： The main morphological characters of this strain were white cotton-like colonies, phialide with inflated base, drastically sharping with partially bending tips, small and transparent budding spores with being always assemble to spearhead and globular, subglobular or ellipse conidiospores. The phylogenetic trees of ITS and 18SrDNA sequences were constructed and analyzed in Tolypocladium. It was resulted that Tolypocladium was confirmed to be monophyletic, and the strain SFYT002 was the same as the systematic position of others of T. inflatum. Meanwhile, the antibacterial test was performed against the 4 common pathogenic bacteria. It was showed that both fermentation and its extracts of different polar from this strain possessed good anti-bacteria capacities. Conclusion： The strain SFYT002 was identified as T. inflatum, and inhibited effectively growth of bacteria.

关 键 词：细虫草 膨大弯茎霉 鉴定 抑菌 新疆 

分 类 号：S567.35[农业科学—中草药栽培]