多发性骨髓瘤患者PBMC中miR-335的甲基化及其表达研究  

Investigation on the methylation and expression of miR-335 in PBMC from the patients with multiple myeloma

作　　者：吴茵[1] 戚菁[2] 吴信华[2] 申娴娟[2] 施维[2] 郝天波 唐瑶瑶[1] 鞠少卿[3] 

机构地区：[1]南通大学医学院,江苏南通226001 [2]南通大学附属医院外科综合实验室,江苏南通226001 [3]南通大学附属医院检验医学中心,江苏南通226001

出　　处：《临床检验杂志》2014年第1期34-37,共4页Chinese Journal of Clinical Laboratory Science

基　　金：国家自然科学基金(81271920);江苏省创新团队(Lj201133)

摘　　要：目的探讨micorRNA-335(miR-335)表达及甲基化水平在多发性骨髓瘤(MM)诊断中的应用。方法用荧光定量PCR检测43例MM患者和30例体检健康者外周血单个核细胞(PBMC)中miR-335的表达水平,用甲基化特异性PCR(MSP)检测miR-335启动子区甲基化水平,分析其与各临床指标的相关性。结果 43例MM患者PBMC中miR-335的表达水平为34.8(15.3,84.9),显著低于健康对照组的269.4(63.3,780.2),Z=4.384,P<0.05。11例MM患者(25.6%,11/43)呈现完全甲基化,其余患者均为部分甲基化,而23例体检健康者(76.7%)呈现完全非甲基化(Uc=6.62,P<0.05)。初诊MM患者miR-335表达量高于复发/进展期患者(Z=2.309,P<0.05),但复发/进展期患者中miR-335启动子区甲基化程度(75%)高于初诊患者(25%),T=24.5,P<0.05。MM患者miR-335表达水平与轻链λ浓度呈相关性(r=0.51,P<0.05)。结论 MM患者miR-335表达水平和甲基化可作为MM潜在的辅助诊断指标。Objective：To investigate the role of the expression and methylation of micorRNA-335 （miR-335） in the diagnosis of multiple myeloma （MM）. Methods：The expression level and the promoter methylation status of miR-335 in the peripheral blood mononuclear cells （PBMC） from 43 MM patients and 30 healthy controls were detected by SYBR Green I QF-PCR and methylation specific PCR （MSP）, respectively, and their correlations with serum IgG, LDH, β2-M and λ chain levels were analyzed. Results：The relative expression level of miR-335 in PBMC from MM patients [34.8（15.3, 84.9）] was significantly lower than that from healthy controls[269.4（63.3, 780.2）, Z=4.384,P〈0.05]. Among 43 MM patients, there were 11 （25.6%） with miR 335 promoter completely methylated, while 23 （76.7%, 23/30） healthy controls showed completely unmethylated （Uc=6.62, P〈0.05）. The relative expression level of miR-335 in first visit patients was significantly higher than that in relapse or progressed patients （Z=2.309, P〈0.05）. However, the promoter methylation level of miR-335 in first visit patients （25%） was significantly lower than that in relapse or progressed patients （75%, T=24.5, P〈0.05）. In addition, the relative expression level of miR-335 in MM patients was correlated with λ chain concentration in serum （r=0.51, P〈0.05）. Conclusion：The expression and methylation levels of miR-335 may be helpful to the diagnosis of MM.

关 键 词：多发性骨髓瘤 miR-335 甲基化 

分 类 号：R733.3[医药卫生—肿瘤]