溶藻细菌L7代谢物对水华鱼腥藻的溶藻效应  

Algicidal Effect of Metabolites of Algicidal Bacteria Strain L7 on Anabaena Flosaquae

作　　者：谢静[1] 潘伟斌[1] 曾嘉韫 

机构地区：[1]华南理工大学环境与能源学院,广东广州510006

出　　处：《环境保护科学》2014年第1期36-40,共5页Environmental Protection Science

基　　金：广州市黄埔区环境保护局资助项目"广州市城区富营养化池塘生态修复示范工程"(302D804212)资助

摘　　要：从等体积不同密度的溶藻细菌L7菌液中提取胞内、胞外代谢物,分别加入等体积等密度的水华鱼腥藻中连续培养7d,研究L7代谢物对水华鱼腥藻的溶藻效应.2.78×10^5～2.78×10^7 cfu/mL的L7胞内代谢物能显著促进藻生长和异形胞分化,到培养结束藻密度为对照组的1.7～1.98倍;异形胞数最大可为对照组的2.31倍.2.78×10^8、2.78×10^9 cfu/mL的L7胞内代谢物能抑制藻生长,藻密度最低为对照组的58％;能极显著抑制异形胞分化,可不形成异形胞.2.78×10^5～2.78×10^9 cfu/mL的L7胞外代谢物能极显著抑制藻生长和异形胞分化,藻密度最低为对照组的0.63％,不形成异形胞;藻丝的藻细胞间隔变模糊,藻细胞变大变形,并有大量的单个球形藻体散落.L7主要通过分泌胞外代谢物溶藻,菌胞内代谢物对藻生长有“低促高抑”的作用.To study the effect of metabolites of algicidal bacteria strain L7 on anabaena flosaquae, intracellular and extracellular metabolites were extracted from equal volume of different densities algicidal bacteria strain L7 broth, then added separately to equal volume and density Anabaena flosaquae culture medium , cultured 7 days continuously. Intracellular metabolites of the 2.78x10%2.78x107 cfu/mL bacteria can stimulate algae growth significantly, at the end of the culture, the density of algal was 1.7-1.98 times of the control group; they can also extremely stimulate heterocyst differentiation significantly, heterocyst can be 2.31 times of the control group. Intracellular metabolites of the 2.78×10^8-2.78×10^9 cfu/mL bacteria can inhibit algae growth, the lowest density of algal can be only 58% of the control group ; they can also extremely inhibit heterocyst differentiation significantly, even no heterocyst formation. Extracellnlar metabolites of the 2.78×10^5-2.78×10^9 cfu/mL bacteria can extremely inhibit algae growth and heterocyst differentiation significantly, the lowest density of algal can be 0.63% of the control group, no heterocyst formation; the intervals between algae cells on the filaments become blurred, algae cells become larger and deformed, with amounts of single algae cells scattered. Algicidal bacteria strain L7 mainly secrete extracellular metabolites to dissolve algaes, intracellular metabolites of low- density bacteria can stimulate algae growth, while intracellular metabolites of high-density bacteria can inhibit algae growth.

关 键 词：溶藻细菌 代谢物 水华鱼腥藻 溶藻效应 

分 类 号：X17[环境科学与工程—环境科学]