A Dual Ligand Targeted Nanoprobe with High MRI Sensitivity for Diagnosis of Breast Cancer  

作　　者：Fa-ming Gong Zuo-quan Zhang Xiao-dong Chen Lu Zhang Xing-su Yu Qi-hua Yang Xin-tao Shuai 梁碧玲 程度 

机构地区：[1]PCFM Lab of Ministry of Education,School of Chemistry and Chemical Engineering,Sun Yat-sen University [2]Department of Radiology,Sun Yat-sen Memorial Hospital,Sun Yat-sen University [3]Department of Radiology,The Fifth Affiliated Hospital,Sun Yat-sen University [4]Center of Biomedical Engineering,Zhongshan School of Medicine,Sun Yat-Sen University

出　　处：《Chinese Journal of Polymer Science》2014年第3期321-332,共12页高分子科学（英文版）

基　　金：supported by the 863 Programs of China(No.2009AA03Z310);National Natural Science Foundation of China(Nos.21174166,30973419);the Ph.D.Programs Foundation of Ministry of Education of China(No.20100171110011);the Postdoctoral Foundation(No.201003370);Natural Science Foundation(Nos.9351027501000003,S2011020003140);S&T Programs of Guangdong Province(Nos.2010B031500011,2009B030803003,2009B030801107,2012B031800135);SYSU Projects for Promotion of Key and Emerging Interdisciplinary Researches(10ykjc18);Young Teachers(11lgpy44)

摘　　要：Antibody targeted delivery is an effective strategy to improve the diagnostic imaging outcome of nanoscale imaging agents in the focal areas. Dual targeting micelles encapsulating superparamagnetic iron oxide were prepared from the amphiphilic block copolymer poly（ethylene glycol）-poly（e-caprolactone） （PEG-b-PCL） with different targeting ligands cRGD and scFv-ErbB single chain antibody conjugated to the distal ends of PEG block. The breast cancer animal model was established by subcutaneous injecting the BT474 cells into the BALB/c-nu female nude mice and then employed to assess the potential of the dual ligand targeted magnetic micelles as a novel MRI contrast agent on a 1.5 T clinical MR/scanner. The T2 signal intensity of the tumor in animals receiving the dual ligand targeted magnetic micelles via tail vein decreased more significantly than the single ligand targeted and nontargeted magnetic micelles. These results indicate that the dual ligand targeted magnetic micelles, cRGD/scFv-ErbB-PEG-PCL-SPION, have great potential to act as a new type of effective nanoscale MRI contrast agent for early diagnosis of breast cancer.Antibody targeted delivery is an effective strategy to improve the diagnostic imaging outcome of nanoscale imaging agents in the focal areas. Dual targeting micelles encapsulating superparamagnetic iron oxide were prepared from the amphiphilic block copolymer poly（ethylene glycol）-poly（e-caprolactone） （PEG-b-PCL） with different targeting ligands cRGD and scFv-ErbB single chain antibody conjugated to the distal ends of PEG block. The breast cancer animal model was established by subcutaneous injecting the BT474 cells into the BALB/c-nu female nude mice and then employed to assess the potential of the dual ligand targeted magnetic micelles as a novel MRI contrast agent on a 1.5 T clinical MR/scanner. The T2 signal intensity of the tumor in animals receiving the dual ligand targeted magnetic micelles via tail vein decreased more significantly than the single ligand targeted and nontargeted magnetic micelles. These results indicate that the dual ligand targeted magnetic micelles, cRGD/scFv-ErbB-PEG-PCL-SPION, have great potential to act as a new type of effective nanoscale MRI contrast agent for early diagnosis of breast cancer.

关 键 词：Block copolymer micelle Superparamagnetic iron oxide （SPI0） Breast cancer Magnetic resonance imaging（MRI） HERCEPTIN c（Arg-Gly-Asp-d-Phe-Lys） （cRGDfK）. 

分 类 号：R737.9[医药卫生—肿瘤]