与感觉相关的ASIC3、TRPV1基因敲除小鼠繁殖性能的观察与痛阈的比较  

The reproductive performance and the pain threshold in ASIC3^(-/-) and TRPV1^(-/-) mice

作　　者：石宏[1] 吉长福[1] 尚红燕[1] 辛娟娟[2] 杨兆坤[1] 宿杨帅[1] 王晓宇[1] 何伟[1] 景向红[1] 

机构地区：[1]中国中医科学院针灸研究所,北京100700 [2]山东中医药大学,济南250355

出　　处：《中国比较医学杂志》2013年第5期1-4,共4页Chinese Journal of Comparative Medicine

基　　金：国家自然科学基金项目(81173205);国家重点基础研究发展计划(973计划)(2011CB505201)

摘　　要：目的观察酸敏感离子通道亚基3(ASIC3,又名ACCN3)基因敲除ASIC3-/-小鼠、香草酸瞬时受体亚型I(TRPV1)基因敲除TRPV1-/-小鼠的繁殖性能,并对其基础痛阈值进行测定和比较。方法选用成熟的未交配过的ASIC3-/-和TRPV1-/-纯合子小鼠分别进行繁殖交配,统计两种小鼠的妊娠率、窝产仔数、离乳率、头胎产仔数等,并对两种小鼠的基础痛阈值进行测定比较,对照组为同源野生C57BL/6小鼠。结果 ASIC3-/-小鼠的妊娠率、窝产仔数、离乳率较TRPV1-/-小鼠偏低,而TRPV1-/-小鼠妊娠率、窝产仔数、离乳率相对稳定;ASIC3-/-组的基础机械痛阈值与C57BL/6对照组相比显著升高(P<0.001),而热痛阈值没有明显改变;TRPV1-/-组的基础热痛阈值与C57BL/6对照组显著升高(P<0.001)。结论ASIC3-/-小鼠的繁殖能力较TRPV1-/-小鼠弱;ASIC3-/-小鼠的机械痛敏感性下降,TRPV1-/-小鼠的热痛敏感性下降。Objective To investigate the reproductive performance and compare pain threshold of ASIC3-/- and TRPV1 -/- mice with C57BL/6 mice. Method 12 ASIC3-/- mice, 12 TRPV1 -/-mice and 12 C57BL/6 mice were chosen and monogamously mated. There are 6 male and 6 female mice in each group, with age of 8 - 10 weeks. The pregnancy rate, the litter size, and the weaning rate of the ASIC3 -/- mice and TRPV1 -/- mice were analyzed. The pain threshold among C57BL/6 mice, ASIC3 -/- mice and TRPV1 -/-mice were compared. Result The pregnancy rate, the litter size, and the weaning rate of the ASIC3 -/- mice is slower than that of the TRPV1 -/- mice. Comparing with C57BL/6 mice,the tactile threshold of ASIC3-/- mice was significantly higher （P 〈0. 001 ） and the thermal threshold of TRPV1 -/- mice was significantly higher （P 〈 0. 001 ）. Condusion The reproductive performance of the ASIC3-/- mice is weaker than that of the TRPV1-/- mice. The tactile sensitivity of the ASIC3-/- mice decreased. And the thermal sensitivity of the TRPV1 -/- mice decreased.

关 键 词：ASIC3- - TRPV1- - 小鼠 繁殖性能 基因敲除 痛阈 

分 类 号：R-332[医药卫生]