无呼吸机支持下小鼠主动脉弓缩窄模型优化法评价  

An optimized method of transverse aortic constriction in mice without ventilator

作　　者：张焕基[1,2] 黄辉[1] 张坤[1] 刘宇[1] 黄菲菲[1] 王景峰[1] 

机构地区：[1]中山大学孙逸仙纪念医院心内科,广州510120 [2]深圳市第四人民医院心内科,深圳510083

出　　处：《中国比较医学杂志》2013年第5期19-22,共4页Chinese Journal of Comparative Medicine

基　　金：国家自然基金项目;编号81270212

摘　　要：目的研究在无呼吸机支持下小鼠主动脉弓缩窄模型优化法,比较其与传统模型构建方法在手术存活率方面的差别。方法取雄性C57BL/6小鼠90只进行TAC模型建立,分为假手术组30只,无呼吸机手术组30只,呼吸机手术组30只,比较3组术后1周总存活率。结果假手术组无1只死亡;无呼吸机手术组术中死亡4只,术后1周死亡0只,总存活率86.7%;呼吸机手术组术中死亡9只术后1周内死亡2只,总存活率63.3%。无呼吸机手术组和呼吸机手术组比较差异有统计学意义(P=0.037<0.05)。两手术组术后4周的心脏超声检测均获得理想的心肌肥厚模型。结论优化后的无呼吸机支持下小鼠主动脉弓缩窄模型的手术方法较传统方法手术存活率更高,手术方式更为便捷。Objective To compare an optimized method of transverse aortic constriction （TAC） without ventilator and TAC with ventilator by the survival rate of mice in one week. Methods Ninety C57BL/6 mice were randomly divided into sham-operated group （ n = 30 ） , TAC without ventilator group （ n = 30 ） and TAC with ventilator group （n = 30 ）. The survival rates were recorded in 1 week and the cardiac function was evaluated by using ultrasonic instrument 4 weeks after the operation. Results The mice survival rate was higher in the TAC group without ventilator than in that with ventilator, and the new improved method resulted in the same cardiac hypertrophic model after 4 weeks. Conclusions The new optimized method of TAC without ventilator is better for improving survival rate and more convenient than the traditional TAC with ventilator.

关 键 词：主动脉弓缩窄模型 呼吸机 小鼠 优化法 

分 类 号：R-332[医药卫生]