大鼠经寰枢椎蛛网膜下腔置管法与传统置管法的比较  

Comparison of the atlantoaxial subarachnoid catheterization in rat with the traditional catheterization

作　　者：陈锐[1] 王华[1] 江伟[1] 

机构地区：[1]上海交通大学医学院附属第六人民医院麻醉科,上海200233

出　　处：《中国比较医学杂志》2013年第7期32-35,共4页Chinese Journal of Comparative Medicine

基　　金：国家自然科学基金--青年支持(81100825)

摘　　要：目的介绍一种新型大鼠蛛网膜下腔置管法,并比较几种常见的鞘内置管方法,为脊髓水平的神经生理实验及疼痛研究提供更有效方案。方法选择90只230～250 g雄性SD大鼠,随机分为Yaksh组,寰枢椎(AA)组和尾置管(L)组,分别从枕骨大孔、寰枢椎间隙、腰5-6椎间隙置管至蛛网膜下腔。三组模型建立后,观察大鼠置管后死亡率、肢体瘫痪率及体重变化情况,并测定其缩足反应阈值(PWT)、甩尾反应潜伏期(TF)和吗啡镇痛效果。对比三组间是否有差异。结果置管后AA组和L组发生死亡和肢体瘫痪的百分率明显低于Yaksh组;且体重恢复情况明显优于Ysksh组;术后7d内,AA组大鼠的PWT值和TF值与术前基础值相比最为接近;吗啡镇痛实验说明AA组置管方法与其他组相比并无差异。结论大鼠经寰枢椎蛛网膜下腔置管方法具有死瘫率低、身体恢复快、对后续实验影响小等优点,是一种较好的新型鞘内置管方法。Objective To establish a new catheterization of the spinal subarachnoid space in rats and to compare the difference between different types of intrathecal catheterization, which can be used for the research on neurophysiology and pain treatment. Methods ninty male SD rats weighing 200 - 250g were randomly divided into three groups with thirty animals in each group： Yaksh group, atlas and axis group （AA group） and lumbar group （L group）. After the models were established, we observed the percentage of death and paralysis of limbs and body weight changes; we also measured paw withdrawal threshold （PWT）, tail flick latency and analgesic effect of morphine. Results The percentage of death and paralysis of limbs in AA group and L group were obviously lower than Yaksh group, and the weight recovered faster than Yaksh group. Within 7 days of catheterization, PWT and the tail flick latency of AA group was closest to baseline. Morphine analgesic experiment showed there was no difference among the three groups. Conclusion The atlantoaxial catheterization model of the spinal subarachnoid space is characterized by its higher duplicability and less injury, which was a good intrathecal catheterization model.

关 键 词：鞘内置管 寰枢椎 动物模型 

分 类 号：R-332[医药卫生]