同品系小鼠卵巢移植方法的建立  

Established a method of ovarian heterotopically transplantation in same strain

作　　者：郁丽丽 刘丽均 王俊风 魏勤[2] 李淼[2] 张艺宝[3] 施美莲 廖侃[2] 徐平[1,2] 

机构地区：[1]上海斯莱克实验动物有限责任公司,上海201615 [2]中国科学院上海生命科学研究院,上海200031 [3]南京农业大学动物医学院,南京210006

出　　处：《中国比较医学杂志》2013年第7期41-43,I0004,共4页Chinese Journal of Comparative Medicine

基　　金：上海市科委科技项目(11140902700);"重大新药创制"科技重大专项(2011ZX09307-302);国家科技支撑计划课题(2013BAK11B02)

摘　　要：目的建立KM、ICR、C57BL/6J、BALB/C四个品系新鲜卵巢异体同位移植的方法。方法上述四个品系出生10 d、20 d、4周的仔鼠作为供体,取出整个卵巢,将卵巢原位移植到同品系4周龄受体雌鼠,术后置于屏障系统内精心饲养,5～8周后,与同品系成年雄鼠交配,观察移植受体是否有正常的繁殖功能。结果同品系不同时间段的新鲜卵巢,移植到同品系受体中,移植卵巢在受体体内可以恢复正常功能,受体可以正常交配,产生正常的仔鼠。结论同品系不同日龄的异体同位卵巢移植方法可行。Objective To establish a method of ovarian heterotopically transplantation in KM, ICR, BALB/c and C57BL/6J mice. Methods the ovaries were used as donor from 10d, 20d and 4w of KM, ICR, BALB/C and C57BL/6J mice, and were grafted to the 4w accepters of same strains. All accepters were bred about 4-week-old, then mated with the same strain male mice to identify the reproductive capacity of the donors. Results the grafted ovaries could be restored reproduction, and gotten the normal pups when mated with same strain males. Conclusion It is feasible that ovarian heterotopically transplantation in the same mouse strains of different age phases.

关 键 词：小鼠 卵巢移植 同位移植 

分 类 号：R-332[医药卫生]