两种不同蛋白质组成类型的花生种子甲硫氨酸含量的比较分析  

Comparative Analysis of Methionine Contents in Two Types of Peanut Seeds

作　　者：芦春斌[1] 黄上志[2] 傅家瑞[2] 

机构地区：[1]暨南大学生殖免疫中心,广州510632 [2]中山大学生物科学与技术系,广州510275

出　　处：《种子》2000年第1期3-5,共3页Seed

基　　金：广东省自然科学基金! (950 0 90 )资助课题

摘　　要：本文对两种不同蛋白质组成类型花生的子叶总蛋白 3个主要组分 ,花生球蛋白、伴花生球蛋白 和伴花生球蛋白 进行了部分纯化 ,并进行了氨基酸分析。它们的氨基酸组成研究表明 ,这两种类型花生的子叶总蛋白中及其 3个主要组分均含有 1 7种氨基酸 ,包括 8种必需氨基酸 ,其中天冬氨酸、谷氨酸和精氨酚含量为最高 ,约为总量的 45 % :而甲硫氨酸含量和半胱氨酸水平都极低。在这 3个主要组分中 ,花生球蛋白的甲硫氨酸含量最低 ,伴花生球蛋白 次之 ,伴花生球蛋白 最高。高甲硫氨酸类型品种 (汕油 5 2 3)其花生球蛋白、伴花生球蛋白 和伴花生球蛋白 的甲硫氨酸含量均显著高于海花The 3 major components of salt soluble proteins were partly purified on Sehadex G 100 gel filtration. Composition of amino acid for urified 3 major components were analyzed. It was showed that 17 amino acids were detectable, included 8 essential amino acids. Asparatic acid, Glutaic acid and Arginine were highest level. Methionine and Cysteine were extremely low level. The methionine level of 3 major components in methionine rich cultivar were obviously higher than that of respective components in low methionine cultivar. The methionine level of Conarachin Ⅱ in 3 major components was the highest in the two cultivars.

关 键 词：花生 种子 贮藏 蛋白 甲硫氨酸含量 氨基酸组成 

分 类 号：S565.201[农业科学—作物学] S565.203.2