光神霉素A抑制人肺腺癌A549/DDP细胞MRP1基因表达  

Mithramycin A Inhibited Expression of MRP1 Gene of Human Lung Adenocarcinoma Cell A549/DDP

作　　者：李怀永[1] 邵淑丽[1] 张伟伟[1] 杨希婷 刘锐[1] 陈闯[1] 车家祥 徐君懿 

机构地区：[1]齐齐哈尔大学生命科学与农林学院,齐齐哈尔161006

出　　处：《中国细胞生物学学报》2014年第3期326-330,共5页Chinese Journal of Cell Biology

基　　金：黑龙江省自然科学基金(批准号:C200624;C201241);黑龙江省教育厅科学技术项目(批准号:11511447;12511611);齐齐哈尔大学研究生创新科研项目(批准号:YJSCX2013-ZD14)资助的课题~~

摘　　要：该研究旨在探讨Sp1抑制剂光神霉素A(Mithramycin A)对人肺腺癌A549/DDP细胞MRP1表达的影响。不同浓度光神霉素A作用A549/DDP细胞48 h后,采用MTT法检测细胞存活率,Real time RT-PCR检测Sp1和MRP1基因表达水平,Western blot检测Sp1和MRP1蛋白表达水平。结果显示,300 nmol/L光神霉素A作用A549/DDP细胞48 h后Sp1和MRP1 mRNA表达水平分别降低31.22%和85.44%,Sp1和MRP1蛋白表达水平分别降低53.27%和40.42%。提示光神霉素A能够通过抑制Sp1表达,从而抑制MRP1表达。The study investigated the effects of Mithramycin A on the expression of MRP1 gene of human lung adenocarcinoma cell A549/DDR The inhibitory effect of Mithramycin A on the growth of A549/DDP cells was tested by MTT method in vitro. The levels of Spl/MRP1 were measured by Real time RT-PCR and Western blot. The results showed that Mithramycin A inhibited the growth of A549/DDP cells in a dose-dependent manner. After exposure to 300 nmol/L Mithramycin A for 48 h, the expressions of mRNA and protein of Spl and MRP1 were reduced. The results suggested that Mithramycin A depressed the expression of MRP1 gene of human lung adenocarcinoma cell A549/DDP through down-regulation of SpI gene.

关 键 词：肺癌 MRP1 SP1 光神霉素A 

分 类 号：R734.2[医药卫生—肿瘤]