人工栽培绣球菌的鉴定及其子实体β‐葡聚糖含量的酶法测定  

Molecular identification of cultivated Sparassis species and determination of β‐glucan content of the fruiting body by using enzymatic method

作　　者：廉添添 杨涛[1] 孙军德[2] 黄贤华 董彩虹[1] 

机构地区：[1]中国科学院微生物研究所真菌学国家重点实验室,北京100101 [2]沈阳农业大学土地与环境学院,辽宁沈阳110866 [3]福建容益菌业科技研发有限公司,福建福州350100

出　　处：《菌物学报》2014年第2期254-261,共8页Mycosystema

基　　金：国家重点基础研究发展计划项目(No.2014CB138302);国家自然科学基金(No.31100014);中国科学院微生物研究所真菌学国家重点实验室资助

摘　　要：在对中国福建省栽培的绣球菌进行种类鉴定的基础上,采用国际通用的酶法测定绣球菌子实体β‐葡聚糖含量,研究了干燥方式对其含量的影响,并与一些常见食药用真菌的β‐葡聚糖含量进行比较。结果显示该栽培绣球菌为广叶绣球菌Sparassis latifolia,子实体柄部和瓣片部分的β‐葡聚糖含量均超过了40%,柄部的含量显著高于瓣片部分,冻干或烘干对其含量没有显著影响。广叶绣球菌β‐葡聚糖含量极显著高于猴头、香菇、蛹虫草和草原黄蘑菇。The ribosomal DNA internal transcribed spacer （rDNA ITS） region was amplified and sequenced to identify the species of artificial cultured Sparassis. The β-glucan content of fruit body of Sparassis was determined and compared with other edible fungi using enzymatic method. The effects of two different drying methods including freeze-drying and hot air-drying on the β-glucan content from Sparassis were also assessed. The results showed that the species of Sparassis cultivated in Fujian Province was S. latifolia. The β-glucan content of both stipes and flabellae of S. latifolia was over 40%, and that of stipes was significantly higher than that of flabellae. There was no significant difference between β-glucan content of freeze-drying fruit body and that of hot air-drying one. It was indicated that the β-glucan content from S. latifolia was significantly higher than that of Hericium erinaceus, Cordyceps militaris, Lentinula edodes and Armillaria luteovirens.

关 键 词：广叶绣球菌 Β-葡聚糖 冷冻干燥 烘干干燥 

分 类 号：S646[农业科学—蔬菜学]