MEF来源的间充质干细胞诱导生成的调节型树突状细胞的基因表达谱分析  

The gene expression profile analysis of regDC induced by MEF-MSC

作　　者：葛超卓[1] 刘星霞[1] 任少达[1] 赵春华[1] 

机构地区：[1]中国医学科学院基础医学研究所组织工程中心,北京100005

出　　处：《基础医学与临床》2014年第4期433-438,共6页Basic and Clinical Medicine

基　　金：国家自然科学基金(30911130363)

摘　　要：目的研究MEF来源的间充质干细胞(MEF-MSC)诱导的调节型树突状细胞(MEF-regDC)与不成熟树突状细胞(imDC)和成熟树突状细胞(maDC)之间的基因表达谱差异,从而揭示前者的基因表达特点。方法将骨髓造血干细胞与MEF-MSC共培养制备MEF-regDC,用Agilent芯片对3利树突状细胞(DC)进行基因芯片检测及相关分析,分析项目包括:差异倍数计算、主成分分析(PCA)和Go分类。结果MEF-regDC具有特征性的形态、表型和基因表达谱,PCA分析表明,MEF-regDC、imDC和maDC是不同的DC亚群,MEF-regDC与imDC相比,表达差异在2倍以上的基因数共有13 369个,差异在6倍以上的有3 247个,MEF-regDC与maDC相比,表达差异在2倍以上的基因数共有11 326个,差异在5倍以上的有3 299个,进一步的分析显示,3者在功能调节相关基因,免疫相关的基因和信号通路相关的基因的表达也有显著差异。结论MEF-MSCs诱导HPCs生成的MEF-regDC是一不同于传统imDC及maDC的具有特征性基因表达谱的DC亚群。Objective To investigate the difference of gene expression between MEF-regDC and imDC ＆ maDC, in order to demonstrate the character of MEF-regDC. Methods We first harvested MEF-regDC by co-culture of bone marrow hematopoietic stem cell with MSC, then analysed 3 types of DCs, including fold change, principal component analysis and Go Term to gene. Results MEF-regDC had the characteristic of morphology, phenotypes and gene expression pro- file, the PCA illustrated that MEF-regDC and imDC ＆ maDC were 3 different type of DC, and there were 13 369 genes that were 2 folds different between MEF-regDC and imDC, and the number of genes that were 6 fold different between the2 types of DCs is 3247; meanwhile, the number of different genes between MEF-regDC and maDC are 11326 and 3299, respectively. Further analysis demonstrated that, there were obvious difference of function regulate genes, immune-relat- ed genes and signaling pathways related genes among these three type of DCs. Conclusions MEF-regDC is a new type of DC which is different from imDC and rnaDC, and has specific gene expression profiles.

关 键 词：树突状细胞 间充质干细胞 基因差异 基因芯片分析 

分 类 号：R329.2[医药卫生—人体解剖和组织胚胎学]