慢病毒介导E2F-1基因过表达抑制人胃癌MGC-803细胞增殖的分子机制  

The molecular mechanisms of human gastric cancer cell line MGC-803 proliferation suppressed by overexpression of E2F-1 mediated by lentivirus

作　　者：曹稳珑 韦尉元[1] 罗文[1] 张笑石 严林海[1] 谢玉波[2] 肖强[1] 

机构地区：[1]广西医科大学第一附属医院胃肠腺体外科,广西南宁530021 [2]广西医科大学第一附属医院麻醉科,广西南宁530021

出　　处：《基础医学与临床》2014年第4期459-463,共5页Basic and Clinical Medicine

基　　金：国家自然科学基金(30860273);广西科学技术攻关项目(桂科攻10124001A-22);广西自然科学基金(2013GXNSFAA019163)

摘　　要：目的探索E2F-1基因过表达抑制人胃癌MGC-803细胞增殖的分子机制。方法用携带E2F-1基因的重组慢病毒颗粒(LV-E2F-1-GFP)感染人胃癌MGC-803细胞,作为实验组(LV-E2F-1-GFP组);以对照慢病毒颗粒(LVGFP)感染人胃癌MGC-803细胞,作为阴性对照组(LV-GFP组);空白对照组常规培养,不做任何处理。用RT-PCR和Western blot技术检测细胞中Skp2、Bax、Bcl-2和survivin基因的表达。结果与LV-GFP组和空白对照组比较,LV-E2F-1-GFP组人胃癌MGC-803细胞Skp2、Bcl-2和survivin基因的mRNA和蛋白的表达降低(P<0.05),Bax基因的mRNA和蛋白的表达上调(P<0.05)。结论慢病毒介导E2F-1基因过表达可能通过降低Skp2、survivin、Bcl-2基因表达和上调Bax基因表达来抑制人胃癌MGC-803细胞增殖。Objective To study the molecular mechanisms of human gastric cancer cell line MGC-803 proliferation suppressed by overexpression of E2F-1. Methods The experimental group （LV-E2F-1-GFP group）, gastric cancer MGC-803 cells were transfected with recombinant lentivirirus vector （LV-E2F-1-GFP）, the negative control group was transfected with negative control lentiviral vector （LV-GFP）. The expression of Skp2, Bax, Bcl-2 and survivin were detected by semi-quantitative RT-PCR and Western blot method. Results Compared with LV-GFP group and blank control group,the mRNA levels of Skp2, Bcl-2 and survivin in the LV-E2F-1-GFP group were reduced significantly （ P 〈 0. 05 ）, the protein levels of Skp2, Bcl-2 and survivin in the LV-E2F-1-GFP group were reduced （ P 〈 O. 05 ）, the mRNA level of Bax and the protein level of Bax were up regulated （P 〈 0. 05 ）. Conclusions The proliferation of human gastric cancer MGC-803 cells is inhibited by overexpression of E2F-1 mediated by lentivirus, the effect maybe explained with down-regulation of Skp2, survivin, Bcl-2 expression and up-regulation of Bax expression.

关 键 词：胃癌 MGC-803细胞 E2F-1 过表达 

分 类 号：R735.2[医药卫生—肿瘤]