骨髓间充质干细胞移植肝衰竭大鼠CD163和白细胞介素10的表达  

Effect of bone marrow mesenchymal stem cells transplantation on the expression of CD163 and interleukin-10 in rats with acute hepatic liver failure

作　　者：袁淑芳[1] 胡兰英[2] 姜涛[3] 孙丽华[1] 郑嵘炅[1] 赵锦艳[2] 张跃新[1] 

机构地区：[1]新疆医科大学第一附属医院感染病中心,新疆维吾尔自治区乌鲁木齐市830054 [2]新疆医科大学第五附属医院,新疆维吾尔自治区乌鲁木齐市830011 [3]新疆医科大学医学动物模型研究重点实验室,新疆维吾尔自治区乌鲁木齐市830054

出　　处：《中国组织工程研究》2014年第6期919-925,共7页Chinese Journal of Tissue Engineering Research

基　　金：新疆医科大学动物模型研究重点实验室开放课题资助项目(XJDX1103-2012-01)~~

摘　　要：背景:研究显示骨髓间充质干细胞具有持久生成肝细胞和胆管细胞的能力,在肝损伤后的修复过程中,参与补充因损伤而减少的肝细胞数量,参与修复因损伤破坏的肝组织结构。目的:探讨骨髓间充质干细胞移植治疗急性肝衰竭大鼠的疗效以及对急性肝衰竭大鼠血清和肝组织CD163、白细胞介素10表达的影响。方法:选取60只SD大鼠,腹腔注射D-氨基半乳糖/脂多糖制备急性肝衰竭模型。随机分为2组,骨髓间充质干细胞移植组尾静脉移植第3代骨髓间充质干细胞,对照组尾静脉注射等体积生理盐水。于移植后24,120,168 h收集血清和肝组织标本。结果与结论:在移植后120,168 h,骨髓间充质干细胞移植组大鼠血清谷丙氨酸氨基转移酶、天冬氨酸氨基转移酶活性显著低于对照组(P<0.05)。骨髓间充质干细胞移植组细胞凋亡指数显著低于对照组(P<0.05)。骨髓间充质干细胞移植组大鼠血清及肝组织中CD163和白细胞介素10水平与对照组比较明显下降(P<0.05),且二者的表达有明显的相关性(P<0.01)。结果可见骨髓间充质干细胞对急性肝衰竭大鼠有一定的治疗作用,CD163、白细胞介素10的基础水平以及治疗过程中的变化趋势可以在一定程度上反映急性肝衰竭的治疗效果。BACKGROUND：Studies have shown that bone marrow mesenchymal stem cells have the ability to persistently generate hepatocytes and biliary cells, and thus in the repair process of liver injury, replenish the reduced number of hepatocytes due to damage and participate in damaged liver structure. OBJECTIVE：To investigate the effect of bone marrow mesenchymal stem cells transplantation on acute liver failure and the expression of CD163 and interleukin-10 in rat serum and liver tissue. METHODS：D-galactose and lipopolysaccharidewere used to make acute liver failure models in 60 Sprague-Dawley rats. Then, the rats were divided into control group and transplantation group. Bone marrow mesenchymal stem cells at passage 3 were injected through tail vein in the transplantation group, and normal saline was injected in the control group. After transplantation 24, 120, 168 hours, serum samples and liver tissues were col ected. RESULTS AND CONCLUSION：After transplantation 120 and 168 hours, the serum alanine transaminase and aspartate aminotransferase activities of the transplantation were significantly lower than those of the control group （P〈0.05）. In the transplantation group the apoptotic index was stil lower compared with the control group, and the difference was significant （P〈0.05）. The levels of CD163 and interleukin-10 in the serum and liver tissue in the transplantation group were decreased significantly compared with the control group （P〈0.05）. The results suggested that there were highly significant correlations between CD163 and interleukin-10 （P〈0.01）. Bone marrow mesenchymal stem celltransplantation has a therapeutic effect on acute liver failure rats. CDl63 and interleukin-10 play a very important role in the pathogenesis of acute liver failure, which can be used as sensitive serum marker proteins for diagnosis and prognosis of acute liver failure.

关 键 词：干细胞 骨髓干细胞 骨髓间充质干细胞 急性肝衰竭 CD163 白细胞介素10 

分 类 号：R394.2[医药卫生—医学遗传学]