基质细胞衍生因子1对内源性神经干细胞的趋化迁移作用  

Stromal cell derived factor 1 effects on migration of endogenous neural stem cells

作　　者：苏稳 丁鹏[1] 王进昆[1] 张浩[1] 牟临杰[1] 王波[1] 刘景传 龚光辉[1] 王崇谦[1] 

机构地区：[1]昆明医学院第一附属医院神经外科,云南省昆明市650032

出　　处：《中国组织工程研究》2014年第6期950-955,共6页Chinese Journal of Tissue Engineering Research

基　　金：2012年云南省卫生科技项目(2012WS0023);项目名称:骨髓基质细胞移植治疗脊髓损伤的超微结构观察~~

摘　　要：背景:目前研究表明,基质细胞衍生因子1在参与趋化迁移内源性神经干细胞中起着非常重要的作用,但其具体迁移机制尚不明确。目的:观察外源性基质细胞衍生因子1对大鼠内源性神经干细胞的趋化迁移作用及海马区神经干细胞的激活增殖情况。方法:通过向SD大鼠海马区上大脑皮质内注射外源性基质细胞衍生因子1(注射量为5μL,质量浓度为500μg/L)建立动物模型,于3,7,14,21 d后灌注取脑,通过石蜡切片免疫组织化学检测大鼠皮质内注射区及海马区nestin阳性细胞表达情况,并设实验对照组与空白对照组。结果与结论:石蜡切片免疫组织化学显示:实验组注射区周围及海马区nestin表达阳性细胞的数量随时间推移逐渐增多,3,7 d时注射区及海马区nestin表达阳性细胞少量表达,14 d时注射区及海马区nestin表达阳性细胞进一步增多,并向注射区形成明显的迁移趋势,21 d时注射区及海马区nestin表达阳性细胞更多。实验对照组及空白实验组未见上述表现。结果表明外源性基质细胞衍生因子1可能诱导海马区神经干细胞的增殖分化,参与内源性神经干细胞的趋化迁移过程。BACKGROUND：Stromal cellderived factor 1 in chemotactic migration of endogenous neural stem cells plays a very important role, but the specific migration mechanism is unclearOBJECTIVE：To observe the effects of exogenous stromal cellderived factor 1 on chemotactic migration and proliferation of neural stem cells in the rat hippocampus METHODS：Exogenous stromal cellderived factor 1 （5μL, 500μ/L） was injected into the hippocampus of Sprague-Dawley rats to establish animal models. Brain tissues were taken after days 3, 7, 14 and 21 of perfusion to prepare paraffin sections. Thereafter, nestin expression in the injection region and hippocampus was detected using immunohistochemical method. Experimental control and blank control groups were set. RESULTS AND CONCLUSION：Paraffin section immunohistochemical results displayed the number of nestin-positive cells in the injection and the hippocampus was gradual y increased. At 3 and 7 days, nestin expression was a little and increased at 14 days, forming a migration tendency to the injection region. At 21 days, there were more nestin-positive cells in the injection area and hippocampus. However, there were no changes as above in the experimental control and blank control groups. The results showed that exogenous stromal cellderived factor 1 may induce the proliferation of neural stem cells in the hippocampus and may be involved in chemotactic migration of endogenous neural stem cells.

关 键 词：干细胞 神经干细胞 干细胞因子及调控因子 基质细胞衍生因子1 内源性神经干细胞 趋化迁移 海马 

分 类 号：R394.2[医药卫生—医学遗传学]