β淀粉样蛋白_(1-42)寡聚体和人重组α-突触核蛋白对原代培养神经元突触的影响  

Effect of β-amyloid_(1-42) and Recombinant Human α-synuclein on Synapses of Primary Cultured Cortical and Hippocampal Neurons

作　　者：王艺璇[1] 喻哲明[1] 丁正同[1] 王坚[1] 邬剑军[1] 陈嬿[1] 

机构地区：[1]复旦大学附属华山医院神经内科,200040

出　　处：《中国临床神经科学》2014年第1期13-19,共7页Chinese Journal of Clinical Neurosciences

摘　　要：目的探讨α-突触核蛋白(α-Syn)和β淀粉样蛋白(Aβ1-42)寡聚体对小鼠原代皮质、海马神经细胞突触功能和活性的影响。方法分别采用二甲基亚砜、Aβ42-1寡聚体、α-Syn、Aβ1-42寡聚体、α-Syn+Aβ42-1寡聚体、α-Syn+Aβ1-42寡聚体干预小鼠原代皮质和海马神经元,按不同干预方法分为6组。干预24 h后用免疫荧光、FM1-43染色法检测神经元的突触数量及活性,同时用Western blot法检测半胱氨酸伸展蛋白α(CSPα)表达。结果与其余5组比较,α-Syn+Aβ1-42寡聚体组可使突触相关CSPα表达明显下降(P<0.01)、与突触数目相关的突触蛋白Ⅰ明显降低(P<0.01),同时突触活性也明显下降(P<0.01)。结论α-Syn和Aβ1-42寡聚体可协同作用于神经元,减少CSPα表达,降低细胞突触数目及其功能。Aim To explore the effect ofα-synuclein （α-syn） and β-amyloid1-42 （Aβ1-42） on the synapses of primary cultured cortical and hippocampal neurons.Methods Primary cultured neurons were divided into 6 groups,each group was treated by DMSO,Aβ42-1,α-syn,Aβ1-42,α-syn＋Aβ42-1 and α-syn＋Aβ1-42,respectively.After 24 hours incubation,the numbers and the activity of synapses were detected by the double immunofluorescence dying and FM 1-43 dying,meanwhile the expression of cysteine string proteinα （CSPα） were analyzed by Western blot.Results Compared with 5 other groups,α-syn＋Aβ1-42 group could significantly decrease the expression of CSPα （P〈0.01）,and synapse-associated synapsin Ⅰ could significantly decrease （P〈0.01）,as well as the synaptic activity（P〈0.01）.Conclusion α-syn together with Aβ1-42 could result in synergistic effect,which would decrease the expression of CSPα and then interact with synapses.The process finally reduced the function and activity of primary cultured cortical and hippocampal neurons.

关 键 词：海马 神经元 突触功能障碍 Α-突触核蛋白 β淀粉样蛋白1-42 β淀粉样蛋白42-1 半胱氨酸伸展蛋白α 

分 类 号：R749.1[医药卫生—神经病学与精神病学]