宫颈癌侧群细胞的恶性行为特征分析  

The Analysis of Malignant Characteristics of Side Population Cells in Patients with Cervical Cancer

作　　者：李晓文[1] 袁俐[1] 张俊平[1] 李建勇[1] 姜忠敏[2] 盛凤[3] 刘晓智[3] 

机构地区：[1]天津市第五中心医院妇产科,300450 [2]天津市第五中心医院病理科,300450 [3]天津市第五中心医院中心实验室,300450

出　　处：《天津医药》2014年第3期211-213,I0004,共4页Tianjin Medical Journal

基　　金：滨海新区卫生局医药卫生科技基金项目(项目编号:2011BHKY007)

摘　　要：目的观察分析宫颈癌患者肿瘤干细胞样侧群细胞的恶性行为特征。方法取4例宫颈癌患者的宫颈癌组织,原代培养宫颈癌细胞,采用流式细胞术分离宫颈癌干细胞样侧群与非侧群细胞,MTT法绘制细胞生长曲线,Transwell培养体系比较两群细胞的侵袭能力,软琼脂克隆实验检测瘤细胞的克隆能力,免疫荧光方法检测耐药基因ATP结合体超家族成员2(ABCG2)的蛋白表达情况;于20只去胸腺小鼠腹股沟皮下接种两群细胞,各10只,比较其体内成瘤率。结果宫颈癌干细胞样侧群细胞占细胞总数的1.39%;与非侧群细胞相比,侧群细胞生长迅速,具有更强的瘤细胞侵袭力和克隆形成能力,且ABCG2的蛋白质表达水平更高。侧群细胞体内成瘤率为100%(10/10),而非侧群细胞为20%(2/10);瘤体病理组织切片可见肿瘤形成,内部伴大片组织坏死。结论宫颈癌肿瘤干细胞样侧群细胞具有更高的恶性细胞表型,是未来宫颈癌基因治疗的核心靶点。Objective To analysis the malignant performance characteristics of tumor stem cell-like side popula-tion cells in patients with cervical cancer. Methods The cervical cancer cells were obtained from surgical resection tumor tissue. The tumor stem cell-like side population cells were isolated by flow cytometry. The cell growth curve was drawn by MTT assay. The invasion ability of tumor cells was compared by transwell assay. The clonogenic capacity was detected by clone formation in soft agar. The expression level of ABCG2 protein, a drug-resistant gene, was detected by immunofluores-cence method. Finally, these cells were transplated into the subcutaneous of de thymus mice. The rate of tumor formation was compared between groups. Results The results from flow cytometry assay showed the percentage of cervical cancer stem cell-like side population cells was 1.39%. Compared with the non-side population cells, the side population cells grow quickly, showed the enhanced invasion ability and colony forming ability. There was more high expression level in ABCG 2 protein of side population cells. The tumor form rate was 100%（10/10） in the side population cells and the non-side popula-tion cells was 20%（2/10）. Conclusion The cervical cancer stem cell-like side population cells have more malignant perfor-mance characteristics than that of non-side population cells, which maybe a core target for cancer gene therapy in the future.

关 键 词：宫颈肿瘤 肿瘤干细胞 侧群细胞 侵袭 耐药性 

分 类 号：R737.3[医药卫生—肿瘤]