荔枝核总黄酮改善胆总管结扎大鼠胆汁淤积症状的研究  

An Experimental Study of Total Flavone from Litchi Chinensis Sonn Improving Symptoms of Cholestatasis in BDL Rats

作　　者：成秋宸[1] 赵永忠[1] 肖绪华[1] 刘瑞彪[2] 黄大健[1] 李胜联[3] 徐庆[4] 

机构地区：[1]桂林医学院附属医院消化内科, 541001 [2]桂林医学院附属医院检验科 ,541001 [3] 桂林医学院公共卫生学院 [4] 桂林医学院药学院

出　　处：《天津医药》2014年第3期224-227,I0006,共5页Tianjin Medical Journal

基　　金：国家自然科学基金资助项目(项目编号:81360659);广西壮族自治区教育厅科研资助项目(项目编号:201012MS172);广西壮族自治区自然科学基金项目(项目编号:2012GXNSFAA053105)

摘　　要：目的观察荔枝核总黄酮(TFL)对胆汁淤积性肝纤维化大鼠肝功能p16蛋白、3型胶原(PC3)和Ⅰ型胶原(PCⅠ)的影响。方法 40只大鼠随机分为假手术(SO)组、胆总管结扎(BDL)组、TFL组和水飞蓟宾胶囊(SIL)组。SO组、BDL组生理盐水灌胃5 mL/(kg·d),TFL组TFL灌胃200 mg/(kg·d),SIL组SIL灌胃5 mL/(kg·d),均持续4周。取血清检测血清丙氨酸转氨酶(ALT)、天冬氨酸转氨酶(AST)、直接胆红素(BILD)、总胆红素(BILT)。取肝组织行HE、Masson染色;Western blot检测p16、PC3、PCⅠ的表达。结果 SO组ALT(IU/L:44.6±8.0)、AST(IU/L:103.8±18.1)、BILD(μmol/L:0.76±0.28)、BILT(μmol/L:1.48±0.35)值最低;BDL组ALT(147.4±86.3)高于TFL组(92.9±47.3),AST(362.7±106.6)高于TFL组(290.1±171.7)和SIL组(250.2±54.9),BILD(99.71±40.87)低于SIL组(137.01±38.86);TFL组BILD(81.48±47.50)、BILT(106.64±61.04)均低于SIL组(均P<0.05)。TFL组存在少量新生胆管,细胞水肿变性不明显,少量炎细胞浸润及胶原沉积,较BDL组肝纤维化程度明显改善。SIL组肝汇管区新生胆管较TFL组明显,有细胞变性水肿,炎细胞浸润,汇管区有纤维间隔,程度较BDL组轻,汇管区有胶原沉积。BDL组p16、PC3、PCⅠ蛋白表达高于TFL组,PC3蛋白低于SIL组,PCⅠ蛋白高于SIL组(均P<0.05),p16蛋白与SIL组差异无统计学意义;TFL组PC16、PC3蛋白表达低于SIL组(P<0.05),PCⅠ蛋白与SIL组差异无统计学意义。结论 TFL可改善胆汁淤积性肝纤维化大鼠肝功能,减轻肝组织纤维化;其机制可能与抑制肝组织p16、PC3、PCⅠ蛋白表达有关。Objective To observe the effects of total flavone from litchi chinensis sonn （TFL） on the liver function in-cluding p16 protein, pro collagen type 3 （PC3） and pro collagen typeⅠ（PCⅠ） in model rats with liver fibrosis induced by bile duct ligation. Methods Forty rats were randomly divided into four groups：sham operation （SO） group, bile duct liga-tion （BDL） group, TFL group and silibinin （SIL） group. Rats were gavaged with saline （5 mL＆#183;kg-1＆#183;d-1） in SO and BDL group, rats were gavaged with TFL （200 mL＆#183;kg-1＆#183;d-1） in TFL group and rats were gavaged with SIL （5 mL＆#183;kg-1＆#183;d-1） in SIL group for four weeks. The serum levels of alanine aminotransferase （ALT）, aspartate aminotransferase （AST）, bilirubin direct （BILD） and bilirubin total （BILT） were detected in four groups. The liver tissues were stained by HE and Masson methods. The ex-pression levels of p16, PC3 and PCⅠin liver tissues were determined by Western blot assay. Results The serum levels of ALT （44.6 IU/L＆#177;8.0 IU/L）, AST （103.8 IU/L＆#177;18.1 IU/L）, BILD （0.76 μmol/L＆#177;0.28μmol/L） and BILT （1.48μmol/L＆#177;0.35μmol/L） were lower in SO group. There was a higher level of ALT in BDL group （147.4 IU/L＆#177;86.3 IU/L） than that of TFL group （92.9 IU/L＆#177;47.3 IU/L）. The serum level of ALT was higher in AST group （362.7 IU/L＆#177;106.6 IU/L） than that of TFL group （290.1 IU/L ＆#177; 171.7 IU/L） and SIL group （250.2 IU/L ＆#177; 54.9 IU/L）. The serum level of BILD was lower in BDL group （99.71μmol/L＆#177;40.87μmol/L） than that of SIL group （137.01μmol/L＆#177;38.86μmol/L）. The serum levels of BILD and BILT were significantly lower in TFL group （81.48μmol/L＆#177;47.50μmol/L, 106.64μmol/L＆#177;61.04μmol/L） than those of SIL group （P＆lt;0.05）. There were small amount of new bile duct and no obvious cells degeneration, small amount of infiltration of in-flammatory cells and col

关 键 词：荔枝核总黄酮 胆汁淤积性肝纤维化 Ⅰ型胶原蛋白 3型胶原蛋白 P16 PCⅠ PC3 P16 

分 类 号：R575.5[医药卫生—消化系统]