正常口腔黏膜全基因组甲基化  

The genome-wide methylation status of normal oral mucosal

作　　者：张晓云[1] 蒋伟文[1] 

机构地区：[1]上海交通大学医学院附属第九人民医院口腔黏膜科,上海200011

出　　处：《临床口腔医学杂志》2014年第3期136-139,共4页Journal of Clinical Stomatology

基　　金：国家自然科学基金(30872887)

摘　　要：目的:了解正常人口腔黏膜全基因组甲基化状态。方法:应用甲基化DNA免疫沉淀技术检测正常人口腔黏膜全基因组甲基化。结果:正常人口腔黏膜中检测出的甲基化CpG岛占总数的16.4%,其中,位于基因启动子区段的甲基化CpG岛占40.2%,甲基化CpG岛和甲基化基因均主要集中于1、2、16、17、19号染色体,甲基化的基因主要集中在与癌症、MAPK、钙信号相关的通路中。结论:人正常口腔黏膜存在甲基化现象,甲基化CpG岛主要集中在富含基因的染色体上。Objective： To understand the genome-wide methylation status in oral mucosa of healthy people. Method： The oral mucosa of healthy subjects was examined by methylated DNA immunoprecipitation. Result： In normal oral mucosa, methylated CpG islands accounted for 16.4 % of the total CpG islands. Methylated CpG islands mainly located in promoter region （40.2 %）. Methylated CpG islands and methylated genes were focused on chromosome 1,2, 16, 17 and 19. Most likely they are involved in the pathways associated with cancer, MAPK, calcium signals. Conclusion： Methylation presents in hu- man normal oral mucosa and methylated CpG islands are primarily located in the gene-rich chromosomes.

关 键 词：正常黏膜 全基因组甲基化 CPG岛 染色体 

分 类 号：R781.5[医药卫生—口腔医学]