窖蛋白1对大鼠星形胶质细胞糖氧剥夺损伤的影响  

Effect of caveolin-1 on oxygen glucose deprivation-induced astrocyte injury in rats

作　　者：许丽丽[1,2] 谢怡[1,2] 马敏敏[1,2] 肖露露[1,2] 刘加美 蓝文雅[1,2] 姜永军[1,2] 叶瑞东[1,2] 刘新峰[1,2] 

机构地区：[1]南京大学医学院附属金陵医院 [2]南京军区南京总医院神经内科,210002

出　　处：《中华老年心脑血管病杂志》2014年第3期307-310,共4页Chinese Journal of Geriatric Heart,Brain and Vessel Diseases

基　　金：国家自然科学基金(31100784;31171016;31300900)

摘　　要：目的探讨糖氧剥夺(oxygen-glucose deprivation,OGD)对体外培养的大鼠星形胶质细胞窖蛋白1(caveolin-1,Cav-1)的影响。方法原代培养出生24h内SD大鼠大脑皮质星形胶质细胞,分为对照组和OGD组(给予星形胶质细胞6h的OGD和24h的再灌注损伤,建立OGD损伤模型)。用多重免疫荧光的方法检测星形胶质细胞Cav-1表达,实时荧光定量PCR和Western blot的方法检测星形胶质细胞Cav-1表达的变化。根据小干扰RNA(siRNA)的原理,将Cav-1siRNA转染星形胶质细胞,采用CCK-8的方法检测星形胶质细胞活力。结果大鼠大脑皮质星形胶质细胞Cav-1普遍表达。与对照组比较,OGD组星形胶质细胞Cav-1mRNA和蛋白表达量下降(P<0.05)。siRNA转染后,与对照组比较,OGD组星形胶质细胞活力下降(P<0.05)。结论 SD大鼠大脑皮质星形胶质细胞Cav-1表达的下调,导致OGD后细胞损伤加重,提示Cav-1对星形胶质细胞具有保护作用。Objective To study the effect of caveolin-1 （Cav-1） on oxygen glucose deprivation （OGD）-induced injury of in vitro cultured astrocytes in rats.Methods Cerebral cortex astrocytes from 1 day old SD rats were primarily cultured.An OGD model was established.The cells were divided into control group and OGD group.The cells in OGD group were subjected to OGD for 6 h and reperfusion for 24 h.Expression of Cav-1 in astrocytes was detected by immunofluorescence,RT-PCR and Western blot,respectively.Cav-1 siRNA was transfected into astrocytes.The survival rate of astrocytes was assayed with the CCK-8 method.Results The Cav-1 was expressed in astrocytes.The expression level of Cav-1 in astrocytes was significantly lower in OGD group than in control group after siRNA transfection （P〈0.05）.Conclusion Down-regulated Cav-1 expression aggravates OGD-induced astrocyte injury in rats,indicating that Cav-1 can protect astrocytes against OGD-induced injury.

关 键 词：窖蛋白1 星形胶质细胞 RNA 小分子干扰 转染 糖氧剥夺 

分 类 号：R363[医药卫生—病理学]