小鼠诱导性多能干细胞向牙源性上皮细胞分化的实验研究  

Experimental study on differentiation of mouse induced pluripotent stem cells into dental epithelial cells

作　　者：刘丽[1] 李亚静[1] 赵文峰[1] 

机构地区：[1]北京军区总医院口腔科,博士主治医师北京100700

出　　处：《中华老年口腔医学杂志》2014年第1期7-11,共5页Chinese Journal of Geriatric Dentistry

摘　　要：目的:探讨小鼠诱导性多能干细胞(iPS,induced pluripotent stem cell)向牙源性上皮细胞分化的可能性和诱导条件,为牙齿再生提供细胞来源。方法:以成釉细胞无血清条件培养液(ASF-CM)为诱导微环境,定向诱导小鼠iPS细胞向牙源性上皮细胞转化,观察培养后形态改变,细胞免疫荧光及RT-PCR检测成釉蛋白(AMBN,ameloblastin)、釉原蛋白(AMGN,amelogenin)、细胞角蛋白14(CK14,cytokeratin 14)的表达。结果:小鼠iPS细胞在ASF-CM诱导微环境下,成功分化为牙源性上皮样细胞,呈典型的上皮样细胞形态,免疫荧光及RT-PCR结果显示:AMBN、AMGN、CK14表达阳性,对照组表达阴性。结论:ASF-CM提供了适宜的成牙微环境,能够促进小鼠iPS细胞向牙源性上皮细胞分化。Objective： To study the possibility and conditions of differentiation of mouse induced pluripotent stem （iPS） cells into dental epithelial cells in vitro, in order to serve a new source of cells for teeth regeneration. Methods： Mouse iPS cells were induced into dental epithelial cells with ameloblasts serum-free conditioned medium （ASF-CM） as induction condition. The resulting dental epithelial cells were cultured and the morphological changes were observed. Immunofluorescence, RT-PCR were used to detect AMBN, AMGN, CK14 expression. Results： The iPS cells successfully induced to dental epithelial cells with typical epithelioid cell morphology. Immunofluorescence and RT-PCR detection showed that the expression of AMBN 、 AMGN and CK14 was positive. In the group of untreated iPS cell, the expression of AMBN, AMGN, and CK14 was negative. Conclusion： In the induction of ASF-CM, iPS cells could successfully differentiate to dental epithelial cells. ASF-CM provides a suitable microenvironment for teeth regeneration.

关 键 词：诱导性多能干细胞 牙齿再生 微环境 

分 类 号：R783[医药卫生—口腔医学]