中国木霉新记录种俄罗斯木霉的分离鉴定及对马铃薯干腐病菌和黑痣病菌的拮抗作用  

Isolation and identification of Trichordema rossicum,a new record of Trichoderma species in China,and antagonism against pathogens of dry rot and black scurf of potato

作　　者：崔岩[1,2,3] 王蒂[2,3] 柴兆祥[1,2,3] 李金花[1,2,3] 余斌[2,3] 

机构地区：[1]甘肃农业大学草业学院,甘肃兰州730070 [2]甘肃省作物遗传改良与种质创新重点实验室,甘肃兰州730070 [3]甘肃省于旱生境作物学重点实验室,甘肃兰州730070

出　　处：《草业学报》2014年第1期276-282,共7页Acta Prataculturae Sinica

基　　金：国家科技支撑计划(2012BAD06B03);甘肃省科技重大专项(1102NKDA025);现代农业产业技术体系(CARS-10-P18);甘肃省教育厅研究生导师科研资助项目(1002-05)资助

摘　　要：利用稀释平板分离法从甘肃国营条山农场马铃薯连作田植株根围土样中分离到1株真菌菌株,经形态学特征、ITS序列分析将其鉴定为俄罗斯木霉(Trichoderma rossicum),为国内木霉新记录种。将其有伤接种马铃薯块茎,发现其对马铃薯块茎无致腐能力。对峙培养法测定该菌株对马铃薯干腐病优势病菌茄病镰刀菌(Fusarium solani)、接骨木镰刀菌(F.sambucinum)和黑痣病菌(Rhizoctonia solani)的拮抗效果,其拮抗系数均在0.70以上,证明其具有较好生防应用潜力。A Trichoderma strain was obtained via dilution methods from rhizosphere soil of continuously cropped potato in Tiaoshan farm,Jingtai County,Gansu province.Based on morphological characteristics,combined with ITS sequence analysis,this strain was identified as T.rossicum,which is a first record of this Trichoderma species in China.It was not pathogenic to potato tubers after pathogenesis tests via wound inoculations,which showed that it was safe to potato tubers.The antagonism of this strain to target pathogens Fusarium solani,F.sambucinum and Rhizoctonia solani （4 strains in total） was tested via dual-culture,and the inhibition indexes were all above 0.70,showing that there was potential for this strain to be applied as a biocontrol agent.

关 键 词：马铃薯 连作 俄罗斯木霉 鉴定 拮抗作用 

分 类 号：S435.32[农业科学—农业昆虫与害虫防治]