MMP-7-181A/G多态性与大动脉粥样硬化性脑梗死的关联性  

Association of matrix metalloproteinase-7-181A /G polymorphism with risk of ischemic strokes caused by large artery atherosclerosis

作　　者：胡晓飞[1] 朱敏[2] 王凤[1] 金笑平[1] 柯绍发[1] 王皖芬[1] 李卫玲[1] 李彩[1] 朱峰[1] 郑周[1] 

机构地区：[1]浙江省台州医院神经内科,317000 [2]浙江省台州医院公共实验室,317000

出　　处：《中华全科医学》2014年第4期510-512,650,共4页Chinese Journal of General Practice

基　　金：浙江省中医药科技计划(2011ZA111);浙江省台州市市级科技资金(201125332)

摘　　要：目的研究基质金属蛋白酶-7(MMP-7)基因启动子区-181A/G多态性与大动脉粥样硬化性脑梗死(LAA)发病风险的关系。方法收集LAA患者327例,其中易损斑块相关为219例,非易损斑块相关为108例,对照组为因穿支动脉病变所致的脑梗死(PAD)患者187例。采用ELISA法检测所有患者MMP-7血清水平,运用聚合酶链反应-限制性片段长度多态性方法分析所有患者MMP-7-181A/G多态性。结果 LAA组MMP-7血清水平与PSD组相比差异无统计学意义(P>0.05),但易损斑块亚组MMP-7水平较非易损斑块亚组高(P<0.01)。基因多态性的分析发现,LAA组AG+GG基因型及G等位基因频率均较PSD组略高,但差异无统计学意义(OR=1.48,P=0.14和OR=1.50,P=0.12)。而易损斑块亚组AG+GG基因型频率较非易损斑块组明显升高,校正年龄、性别、血脂水平等危险因素后差异仍有统计学意义(OR=2.25,P=0.026)。结论 MMP-7-181A/G多态性可能对脑梗死患者LAA和PSD两个亚型的影响无差异,但可能与存在易损斑块的大动脉粥样硬化性脑梗死亚组的发病风险相关。Objective To explore the association of matrix metalloproteinase-7-181A/G polymorphism with risk of ische- mic strokes caused by large artery atheroselerosis. Methods 327 ischemie stroke patients caused by large artery athero- sclerosis （LAA group） were collected, and the control were 187 stroke patients caused by penetrating artery disease（ PAD group）. According to the detection of vulnerable plaque,the LAA group was divided into vulnerable and non-vulnerable subgroups. Plasma MMP-7 levels were measured by enzyme linked immunosorbent assay（EL1SA）,and MMP-7-181A/G genotypes were determined by polymerase chain reaction-restiction fragment length polymorphim（PCR-RFLP）. Results The differences of plasma MMP-7 levels between LAA and PSD group were not significant, while plasma MMP-7 levels of vulnerable subgroups enhanced as compared to non-vulnerable subgroup（ P 〈 0.01 ）. The frequencies of AG ＋ GG geno- type and G allele in LAA group were a little higher than those of PAD group（ 16.5% vs. 11.8% and 8.6% vs. 5.9% respectively）, but no significant differences were observed （OR = 1.48, P = O. 14 and OR = 1.50, P = 0.12 respectively）. The frequency of AG ＋ GG genotype of patients with vulnerable plaque subgroup was significant higher than no vulnerable plaque patients（ ajusted OR = 2.25, P = O. 026 ）. Conclusion The present findings suggest genetic polymorphism of - 181A/G in the MMP-7 promoter may be associated with ischemic strokes caused by large artery atheroselerosis among pa- tients with vulnerable plaques.

关 键 词：基质金属蛋白酶-7 基因多态性 大动脉粥样硬化 穿支动脉病 脑梗死 

分 类 号：R743.33[医药卫生—神经病学与精神病学]