未成熟脑惊厥性损伤与黑质网状部特异性神经保护的研究  

Immature brain convulsion sex damage and SNR specific nerve protection

作　　者：秦春玲[1] 郝艳秋[1] 

机构地区：[1]哈尔滨医科大学附属第二医院儿内科,150086

出　　处：《中国生育健康杂志》2014年第1期41-44,共4页Chinese Journal of Reproductive Health

摘　　要：目的 探讨未成熟脑对惊厥性损伤的耐受性及可能机制,推测黑质网状部（SNR）特异性屏蔽的神经保护作用. 方法 将幼年SD大鼠（21日龄）和成年SD大鼠（2月龄）腹腔注射青霉素建立惊厥模型,分别称为未成熟组和成熟组,观察大鼠惊厥过程中行为改变.建模成功72 h后断头取脑,取SNR做HE染色进行光镜观察. 结果 （1）两组发作强度比较,差异有统计学意义,未成熟组大鼠惊厥发作程度53％为Ⅲ级,成熟组大鼠惊厥发作程度47％为V级;未成熟组开始惊厥的时间、惊厥时间持续、惊厥发生后开始死亡的时间分别为（9±5）min、（39.3±15.3） min、（40±13）min;成熟组分别为（14±6） min、（16.3±8.2）min、（8±4） min,差异均有统计学意义.（2）HE染色后,未成熟脑黑质神经元细胞核嗜碱性较成熟脑组织强,黑质神经元变性、死亡及丢失,所有未成熟脑组织神经元改变,包括SNR、海马、皮层部位的神经元细胞均较成熟脑组织相应部位的神经元损伤程度轻.结论 SNR成熟度对脑惊厥性损伤具有特异性神经保护作用.Objective To explore seizure-induced brain damage and brain maturation level, with the rat substantia nigra pars reticulata （substantianigra pars retieu late SNR ） playing a ＂gate＂ and ＂shield＂ role in the seizure, and to ex- amine the neuroprotective effect of shielding mechanism and the specificity of SNR in immature brain tolerance convulsive injury. Methods Immature SD rats （21 days old） and adult SD rats （2 month old） were randomly divided into 4 groups： the immature experimental group, immature control group, mature experimental group and mature control group. The experimental ~＇oup was injected with penicillin to establish convulsion model, and were observed for the change of be- havior of epileptic rats. The rat brains was detected by HE method at 72 h after being perfused with formaldehyde and sacri- riced. Results Damage was observed in the immature experimental group and mature experimental group, while less se- vere damage was observed in the immature control group than in the mature control group, with delay in time of damage. The level of injury of the immature experimental group and the mature experimental group were significantly different. Conclusion The maturity of SNR on immature brain convulsive injury has specific protective effect on the nervous system.

关 键 词：未成熟脑 惊厥性损伤 癫痫 黑质网状部 

分 类 号：R743.31[医药卫生—神经病学与精神病学]