干酪乳杆菌与银杏叶提取物对肥胖小鼠血脂及抗氧化功能的影响  

Effects of Lactobacillus casei and Ginkgo biloba extract on blood lipids and anti-oxidation of obese mice

作　　者：温啸[1] 贺大方 倪学勤[1] 张丹[1] 丁艳东[1] 辛金鸽 李利杰[1] 曾东[1] 

机构地区：[1]四川农业大学动物医学院动物微生态研究中心,四川雅安625014

出　　处：《西北农林科技大学学报（自然科学版）》2014年第1期8-12,17,共6页Journal of Northwest A&F University(Natural Science Edition)

基　　金：教育部留学回国人员科研启动基金项目(200835-1);四川省学术带头人培养基金项目

摘　　要：【目的】研究干酪乳杆菌与银杏叶提取物协同的降血脂及抗氧化作用。【方法】将体质量为(20±1.0)g/只的60只健康雌性小鼠随机分成5组,即空白对照组、高脂对照组、干酪乳杆菌组、银杏叶提取物组及干酪乳杆菌与银杏叶提取物混合组,除空白对照组外,其余4组均给予高脂饲料,建立肥胖模型,试验期为30d。试验结束后测定各组小鼠体质量,血清中的总胆固醇(TC)、甘油三脂(TG)、高密度脂蛋白胆固醇(HDL-C)、丙二醛(MDA)浓度及谷胱甘肽过氧化物酶(GSH-Px)和过氧化氢酶(CAT)等的活性。【结果】干酪乳杆菌与银杏叶提取物协同作用,能明显降低肥胖小鼠体质量、TC、TG和MDA,与高脂对照组相比,小鼠体质量降低13.22%,TC和TG降解率分别达到24.48%和20.93%,差异极显著(P<0.01);小鼠血清中HDL-C增加167.85%,差异极显著(P<0.01);小鼠血清中GSH-Px活性提高了13.14%(P<0.05),CAT活性降低了49.01%(P<0.01)。【结论】干酪乳杆菌与银杏叶提取物协同使用,具有控制小鼠体质量、降血脂、促进胆固醇代谢的作用,可用于预防和治疗高脂血症。[Objective] The purpose of this experiment was to study the synergistic effects of Lactoba- cillus casei and Ginkgo biloba extract synergies on lowering blood lipids and anti-oxidation. [Method] 60 mice were randomly divided into five groups：blank control group, high-fat control group,L, casei group, G. biloba extract group,and L. casei and G. biloba extract mixture group. High fat diet was fed to groups except blank control to establish obesity model with a period of 30 days. Body weight, serum total choles- terol （TC）, triglyceride （TG）, high density lipoprotein cholesterol （HDL-C）, content of malondialdehyde （MDA） ,and activities of glutathione peroxidase （GSH-Px） and catalase （CAT） were detected. [Result] Compared with high-fat control group,L, casei and G. biloba extract significantly reduced body weight, TC, TG and MDA by 13.22%,24.48% ,20.93% and 5.98% ,respectively （P〈0.01）. The mouse serum HDL- C was enhanced by 167.85% （P〈0.01）. The activity of GSH-Px was increased by 13.14% （P〈0.05）,and the activity of CAT was decreased by 49.01% （P〈0.01）. [Conclusion] Synergistic taking of L. casei and G. biloba extract synergies could control body weight,lower blood fat, promote cholesterol metabolism, and prevent and treat hyperlipidemia.

关 键 词：干酪乳杆菌 银杏叶提取物 降血脂 抗氧化 

分 类 号：R972.6[医药卫生—药品] S859.799.9[医药卫生—药学]