EMS诱变番茄自交系TTD302A的突变表型鉴定和分析  

Identification and Analysis of Mutant Phenotype Tomato Inbred Line TTD302A Induced with EMS

作　　者：杨建华[1] 崔霞[1] 常培培[1] 李翠[1] 李云洲[1] 梁燕[1] 

机构地区：[1]西北农林科技大学园艺学院,陕西杨凌712100

出　　处：《中国蔬菜》2014年第4期21-28,共8页China Vegetables

基　　金：陕西省科技统筹创新工程计划项目(2011KTCL02-03)

摘　　要：为创制用于番茄遗传育种和基因功能研究的新种质,用0.7%的甲基磺酸乙酯(EMS)浸泡处理TTD302A种子8h,清水冲洗后催芽育苗,定植于塑料大棚中,单株观察、单株留种。对M2群体进行株系和单株的系统观察,表型表现一致的株系混合留种,有表型变异的单株进行单株留种,获得M3种子。另外选取了15份出现典型变异性状的M2材料进行SSR检测。通过对M2群体表型性状的观察,共发现373个变异性状,297个变异单株,总的单株变异频率为7.1%。叶、花、果实和植株的表型变异频率依次为1.5%、2.8%、1.3%和2.4%。SSR分析结果表明9份材料在DNA水平上有变异。To create new tomato （Solanum lycopersicum L. ） germplasm applied both to genetic modified breeding and functional genomics research on tomato, this study treated the seeds of TTD302A with 0.7% EMS for 8 h, then washed them by flowing water. ME seeds were harvested from each plant independently in M~ generation. Afterwards, the agronomic traits and biological characters of each plant were both investigated and recorded in M2 generation at seedling stage, flowering fruit period and mature period, respectively. The leaves DNA of all mutation plants were extracted, of which 15 materials expressing typical variation characteristics were selected for SSR molecular marker detection. Finally we harvested M3 seeds from single plant expressing character mutation separately. Through observation on M2 group characters, we found 373 mutant characters and 297 mutant plants in total. The rate of all M2 phenotype mutation added up to 7.1%. It could be found that the mutation population contained abundant mutation types appearing in leaf, floral organ, fruit and plant. Their mutation rates were 1.5%, 2.8%, 1.3%, 2.4%, respectively. The results of SSR analysis indicated that 9 materials had mutation in DNA level.

关 键 词：甲基磺酸乙酯(EMS) 番茄 自交系 突变体 

分 类 号：S641.2[农业科学—蔬菜学]