完整糖肽质谱解析的研究进展  

Advance in Mass Spectrometry-Based Analysis of Intact Glycopeptides

作　　者：王继峰[1,2] 江静[2] 彭博[2] 钟儒刚[1] 应万涛[2] 钱小红[1,2] 

机构地区：[1]北京工业大学生命科学与生物工程学院,北京100022 [2]北京放射与辐射医学研究所、北京蛋白质组研究中心、蛋白质组学国家重点实验室,北京102206

出　　处：《质谱学报》2014年第2期108-117,共10页Journal of Chinese Mass Spectrometry Society

基　　金：国家重点基础研究“973项目”(2011CB910603);国家高技术研究发展计划(2012AA020203);北京市科技新星计划(Z121107002512014)资助

摘　　要：糖基化是一种普遍存在并且具有重要生物学功能的蛋白质翻译后修饰,与疾病发生、诊断、预后和治疗有密切的关系。基于生物质谱的糖蛋白质结构解析技术迅速发展,其中,将糖链与蛋白质分解开,分别进行糖基化位点的确认、肽段序列分析及糖链结构分析的技术已经趋于成熟。但对于涉及蛋白质与糖链之间连接方式的质谱解析却面临很多困难和挑战。本研究着重于位点特异性完整糖肽的质谱解析技术,确定了该领域的主要研究方向,从质谱技术的离子源、质量分析器、碎裂方式,以及质谱数据解析等方面对近年来完整糖肽的质谱分析技术的发展进行综述。Glycosylation is a common form of protein post-translational modification and plays key roles in biological processes, which is closely related to the occurrence, develop- ment, diagnosis and treatment of various diseases. Although the rapid development of bio- logical mass spectrometry based technology offers great conveniences to identification of glycosylation sites, peptides sequences and glycan structures of glycoproteins respectively, the illumination of the linkage between proteins and glycans and the analysis of the struc- ture of intact glycopeptides still face a lot of difficulties and challenges. Based on literatureresearches, the major directions of the field were determined. This review will focus on MS analysis of the site-specific intact glycopeptides and describes the development and prospects of biological mass spectrometry-based technology for the analysis of intact glycopeptides on many aspects, from ion source and mass analyzers to dissociation modes and the analysis of mass spectrometric data.

关 键 词：糖基化修饰 糖肽 质谱 糖蛋白质组学 

分 类 号：O657.63[理学—分析化学]