樟芝菌粉挥发性成分的HSGC/MS分析及其指纹图谱研究  

Study on Volatile Components in Antrodia Camphorata Powder and Their Fingerprints Analysis by HSGC/MS

作　　者：陈菲[1] 张奉苏[1] 杨念云[1] 刘训红[1] 侯娅[1] 马阳[1] 

机构地区：[1]南京中医药大学药学院,江苏南京210023

出　　处：《质谱学报》2014年第2期149-157,共9页Journal of Chinese Mass Spectrometry Society

基　　金：江苏省"药用生物资源研究与利用"优秀科技创新团队支持计划(2011);江苏高校优势学科建设工程项目(ysxk-2010)资助

摘　　要：采用HSGC/MS法分析不同批次、不同培植方法、不同厂家樟芝中的挥发性成分,用峰面积归一法测定各成分的相对百分含量;以2-甲基丙醛为参照物,测定其指纹图谱,并做相似度评价。在台湾普通樟芝菌粉、台湾三萜含量较高樟芝菌粉、樟芝胶囊、椴木栽培樟芝和广东樟芝菌粉中,分别分离鉴定出39种、34种、35种、27种、41种化合物,其中共有成分13种;初步建立了以11个共有峰为特征指纹信息的樟芝菌粉HSGC/MS指纹图谱。The volatile components in Antrodia camphorata of different batches,different cultivation methods and different manufacturers were separated and identified by headspace gas chromatography-mass spectrometry （HSGC/MS） and their fingerprints were established.The contents of the components were determinated by area normalization method.2-methyl-propanal was used as a reference standard,the chromatographic fingerprint was determined,and the degree of different fingerprint patterns was calculated to compare the similarity of samples.39,34,35,27 and 41 volatile components are identified in Taiwan Antrodia camphorata powder,Taiwan Antrodia camphorata powder with higher triterpenoid content,Antrodia camphorata capsule,the basswood cultivated Antrodia camphorata sporophore and Guangdong Antrodia camphorata powder,respectively.There are 13 common components in the five kinds of Antrodia camphorata.HSGC/MS fingerprints with 11 common peaks of Antrodia camphorata powder were established preliminarily.The method provides the valuable materials for quality assessment of Antrodia camphorata powder.

关 键 词：樟芝菌粉 挥发性成分 顶空气相色谱-质谱(HSGC MS) 指纹图谱 

分 类 号：O657.63[理学—分析化学]