橄榄油脂肪乳对LPS诱导急性肺损伤SD幼年大鼠前炎症因子TNF-α、IL-1β、IL-6的影响  

Effect of Clinoleic 20% on the expression of pro-inflammatory cytokines TNF-α,IL-1β and IL-6 in young Sprague-Dawley rat models of lipopolysaccharide-induced acute lung injury

作　　者：石计朋[1] 黄丽密[2] 钱燕[2] 高俊[1] 

机构地区：[1]新乡医学院第一附属医院新生儿科,河南新乡453100 [2]温州医科大学附属第一医院新生儿科

出　　处：《实用预防医学》2014年第3期275-277,260,共4页Practical Preventive Medicine

基　　金：浙江省重点扶植学科"临床营养学"(2007-F03)

摘　　要：目的分析橄榄油脂肪乳对脂多糖(LPS)诱导的急性肺损伤大鼠(ALI)TNF-α、IL-1β和IL-6分泌的影响。方法 60只Sprague-Dawley幼年大鼠按随机数字分为Control组(生理盐水+生理盐水)、LPS组(生理盐水+LPS)和Clino组(20%脂肪乳克林诺+LPS)。各组分别采用生理盐水或橄榄油脂肪乳剂处理7 d;均在取标本前8 h于气管内滴入生理盐水或脂多糖,建立急性肺损伤(ALI)大鼠模型;观察各组大鼠肺组织的病理改变,测定肺泡灌洗液TNF-α、IL-1β、IL-6的表达。结果 (1)ALI模型组大鼠肺组织病理切片均可见明显炎症细胞浸润和出血;(2)ALI模型组肺系数、病理评分均高于Control组;(3)BALF中Clino组TNF-α、IL-1β和IL-6蛋白水平低于LPS组,差异有统计学意义(P<0.05)。结论橄榄油脂肪乳能够通过降低前炎症因子TNF-α、IL-1β和IL-6的分泌从而减轻ALI大鼠炎症反应及肺部损伤。Objective To analyze the effect of Clinoleic 20 % （olive oil-based,n-9） lipid emulsion on the expression of tumor necrosis factor alpha （TNF-α）,interleukin beta （IL-1β） and interleukin 6 （IL-6） in acute lung injury （ALI） rat models induced by lipopolysaccharide （LPS）.Methods Sixty young Sprague-Dawley rats were randomly divided into control group （0.9% NaCl ＋ 0.9% NaCl）,LPS group （0.9% NaCl ＋ LPS）,and Clino group （Clinoleic 20% ＋ LPS）.All rats were infused for seven days with normal saline or different fat emulsions respectively,and sacrificed at 8 hours after intra-tracheal introduction of normal saline or LPS.The pathologic changes of lung tissues of rats in each group were observed; meanwhile,the expression levels of TNF-α,IL-1β and IL-6 in bronchoalveolar lavage fluid （BALF） were measured by enzyme-linked immunospecific assay （ELISA）.Results Pulmonary histopathological alterations：inflammatory cells infiltrating around the bronchus and the vascular,even bleeding and more apoptosis cells were observed in the lungs of rats in ALI model groups.The lung indexes and pathological scores of rats in ALI model groups were both higher than those in the control group.The expression levels of TNF-α,IL-1 β and IL-6 in BALF of the Clino group were all lower than those in BALF of the LPS group,and the difference was statistically significant （P 〈 0.05）.Conclusions Clinoleic 20 % can relieve inflammation and lung injury by downregulating the expression of TNF-α,IL-1β and IL-6.

关 键 词：橄榄油脂肪乳 急性肺损伤 肿瘤坏死因子α 白介素1Β 白介素6 

分 类 号：R-332[医药卫生]