产菊粉酶细菌菌株的筛选及菌种鉴定  

Screening and Identification of Bacterium Producing Inulinases

作　　者：张扬[1] 朱启忠[1] 张瑞[1] 

机构地区：[1]山东大学海洋学院,山东威海264209

出　　处：《资源开发与市场》2014年第4期392-393,400,共3页Resource Development & Market

基　　金：国家支撑项目(编号:2011BAC02B04);山东大学研究生创新项目(2012年度)

摘　　要：从山东威海沿海滩涂菊芋生长的根际土壤中分离得到能产菊粉酶的菌株20株,经过进一步摇瓶复筛,获得了产菊粉酶活力较高的一株细菌F1,其发酵液酶活达到1.73U/mL。通过形态学观察、16SrDNA序列分析及生理生化研究,鉴定该菌为芽孢杆菌属的巨大芽孢杆菌(bacillus megaterium)。20 strains capable of producing inulinase were isolated from rhizosphere soil of jerusalem artichoke in the salt- affected lands of Wei- hai, Shandong Province. A strain of bacteria F1 with high inulinase productivity was obtained through the further screening. The results showed that the enzyme activity was 1.73 U/mL respectively. This strain was identified as bacillus megaterium through morphologic observation, molecular identifica- tion by 16S rDNA sequence analysis and physiological and biochemical studies.

关 键 词：菊粉酶 筛选 菌种鉴定 

分 类 号：Q93-331[生物学—微生物学]