肌筋膜经线选穴法针刺治疗对脑性瘫痪模型大鼠神经细胞再生及运动功能恢复的影响  

Effects of Acupuncture Based on Acupoint Selection of Myofascial Meridians Perspective on Regeneration of Nerve Cells and Recovery of Motor Function in Rats with Cerebral Palsy

作　　者：徐冬晨[1] 杨江 喻斌[3] 李达玲[3] 韩新民[4] 

机构地区：[1]南京特殊教育职业技术学院康复科学系,江苏南京市210000 [2]南京中医药大学附属医院儿科,江苏南京市210000 [3]南京中医药大学药学院,江苏南京市210000 [4]南京中医药大学第一临床医学院,江苏南京市210000

出　　处：《中国康复理论与实践》2014年第3期206-210,共5页Chinese Journal of Rehabilitation Theory and Practice

基　　金：南京中医药大学中医儿科学重点学科开放课题资助项目(No.EZK2012011);江苏高校"青蓝工程"资助项目

摘　　要：目的探讨基于肌筋膜经线的选穴法针刺治疗对脑瘫模型大鼠神经细胞再生及运动功能恢复的影响。方法健康21日龄雄性Sprague-Dawley大鼠20只,分成对照组、针刺1组、针刺2组和假手术组,对照组、针刺1组和针刺2组采用左侧颈总动脉结扎结合低氧环境制作脑瘫模型。针刺1组接受传统针刺疗法,针刺2组接受基于肌筋膜经线的选穴法针刺治疗。各组于针刺开始后第3、7、14天行BBB评分、斜板试验;第14天用5-溴脱氧尿核苷(BrdU)标记检测左脑运动皮层和纹状体的细胞增殖情况。结果第7天和第14天针刺2组的BBB评分恢复明显优于针刺1组和对照组(P<0.01),第3、7、14天针刺2组斜板支撑能力均较对照组升高(P≤0.05);第14天针刺2组运动皮层BrdU含量较针刺1组升高(P<0.05)。结论基于肌筋膜经线的选穴法针刺治疗能有效促进脑瘫模型神经细胞再生和运动功能的改善,且优于传统针刺疗法。Objective To investigate the effects of acupuncture based on acupoint selection of myofascial meridians perspective on the regeneration of nerve cells and recovery of motor function in rats with cerebral palsy. Methods 20 healthy 21-day-old male Sprague-Dawley rats were divided into control group, acupuncture groups 1 （receiving traditional acupuncture） and 2 （receiving acupuncture based on acu-point selection of myofascial meridians perspective）, and sham group. The control group and the acupuncture groups were subjected to liga-tion of left carotid artery （ischemia） and then put into a hypoxic environment. They were assessed with Basso-Beattie-Bresnahan （BBB） Scale and inclined plane test 3 d, 7 d and 14 d after the beginning of acupuncture. And 5-bromo-2＆#39;-deoxyuridine （BrdU） was used to label S-phase cells in the left striaturn and motor cortex in each group 14 d after the beginning of acupuncture. Results The BBB scores improved more in the acupuncture group 2 than in the acupuncture group 1 and the control group 7 d, 14 d after the beginning of acupuncture （P〈0.01）, as well as the score of inclined plane test 3 d, 7 d, 14 d after acupuncture （P〈0.05）. BrdU was more in the left motor cortex in the acu-puncture group 2 than in the acupuncture group 1 （P〈0.05）. Conclusion Acupuncture based on acupoint selection of myofascial meridians perspective can promote the regeneration of nerve cells and recovery of motor function in rats with cerebral palsy, that is more effective than traditional acupuncture.

关 键 词：脑性瘫痪 肌筋膜经线 针刺 穴位 5-溴脱氧尿核苷 运动功能 大鼠 

分 类 号：R742.3[医药卫生—神经病学与精神病学]