Calcium phosphate nanoparticles show an effective activation of the innate immune response in vitro and in vivo after functionalization with flagellin  

作　　者：Diana Kozlova Viktoriya Sokolova Maohua Zhong Ejuan Zhang Jingyi Yang Wei Li Yi Yang Jan Buer Astrid Maria Westendorf Matthias Epple Huimin Yan 

机构地区：[1]Institute of Inorganic Chemistry and Center for Nanointegration Duisburg-Essen(CeNIDE),University of Duisburg-Essen [2]Mucosal Immunity Research Group,State Key Laboratory of Virology,Wuhan Institute of Virology,Chinese Academy of Sciences [3]Institute of Medical Microbiology,University Hospital Essen,University of Duisburg-Essen

出　　处：《Virologica Sinica》2014年第1期33-39,共7页中国病毒学（英文版）

基　　金：supported by the Deutsche Forschungsgemeinschaft(SFB/Transregio 60);the National Natural Science Foundation of China(no.81302609 and 81202312)

摘　　要：For subunit vaccines,adjuvants play a key role in shaping the magnitude,persistence and form of targeted antigen-specific immune response.Flagellin is a potent immune activator by bridging innate inflammatory responses and adaptive immunity and an adjuvant candidate for clinical application.Calcium phosphate nanoparticles are efficient carriers for different biomolecules like DNA,RNA,peptides and proteins.Flagellin-functionalized calcium phosphate nanoparticles were prepared and their immunostimulatory effect on the innate immune system,i.e.the cytokine production,was studied.They induced the production of the proinflammatory cytokines IL-8 （Caco-2 cells） and IL-1β（bone marrow-derived macrophages; BMDM） in vitro and IL-6 in vivo after intraperitoneal injection in mice.The immunostimulation was more pronounced than with free flagellin.For subunit vaccines,adjuvants play a key role in shaping the magnitude,persistence and form of targeted antigen-specific immune response.Flagellin is a potent immune activator by bridging innate inflammatory responses and adaptive immunity and an adjuvant candidate for clinical application.Calcium phosphate nanoparticles are efficient carriers for different biomolecules like DNA,RNA,peptides and proteins.Flagellin-functionalized calcium phosphate nanoparticles were prepared and their immunostimuiatory effect on the innate immune system,i.e.the cytokine production,was studied.They induced the production of the proinflammatory cytokines IL-8(Caco-2 cells) and IL-1β(bone marrow-derived macrophages;BMDM) in vitro and IL-6 in vivo after intraperitoneal injection in mice.The immunostimulation was more pronounced than with free flagellin.

关 键 词：calcium phosphate NANOPARTICLE IMMUNOSTIMULATION innate immune system FLAGELLIN ADJUVANTS 

分 类 号：R392[医药卫生—免疫学]