血管抑素基因治疗Lewis肺癌移植瘤小鼠的实验研究  

Cationic Liposomes Complex Plasmids Encoding Angiostatin Inhibit Lewis Lung Cancer in Mice Model

作　　者：李真斌[1] 齐协飞[2] 

机构地区：[1]江西省胸科医院,江西南昌330008 [2]南昌大学第二附属医院

出　　处：《中国医学创新》2014年第9期26-28,共3页Medical Innovation of China

基　　金：江西省科技厅基金资助项目(200210300101)

摘　　要：目的:观察血管抑素基因对Lewis肺癌小鼠移植瘤生长、转移的抑制作用。方法:建立C57BL/6j小鼠肺癌模型,30只荷瘤鼠按照随机数字表法分为生理盐水空白对照组、阳离子脂质体对照组、血管抑素基因(PAng)治疗组,每组10只。以阳离子脂质体为介导,将血管抑素基因的重组质粒直接注入移植瘤内,每周3次,共5周,每周测瘤体大小1次,第7周末处死所有小鼠,观察瘤体大小变化、肺表面转移灶数、小鼠生存期等。结果:治疗组小鼠移植瘤生长速度减慢,瘤体缩小,肺内转移少,与对照组比较差异有统计学意义(P<0.05),小鼠活动能力、饮食、对外界刺激的反应能力均无明显改变,生存期明显长于对照组(P<0.05)。结论:阳离子脂质体介导血管抑素基因可有效地抑制Lewis肺癌移植瘤的生长、转移。Objective：To explore the inhibitory effect on the growth and metastasis of tumor by cationic liposomes complex plasmids encoding angiostatin in treatment of mice Lewis lung cancer model. Method：The Lewis lung cancer cell were implanted into 30 C57BL/6j mice to established mice model,and then divided them into the normal saline control group,the cationic liposome group,the PAng group,10 rats in each group. Cationic liposome complex plasmids encoding angiostatin were injected into tumor to inhibit the growth and metastasis of the tumor,3 times a week,for 5 weeks,tumors size measurement 1 time a week,all the mice were sacrificed at the end of the seventh week. The size change of the tumor, the number of long surface metastases,survival period of the mice were observed.Result：The treatment group could inhibit the growth and metastasis of the implanted tumor. Comparing with the control group,they showed significance in tumor size, metastasis in lung and survival period of the mice（P〈0.05）. Mice activity,diet,and responses to external stimuli ability had no obvious change,and the lifetime of the treatment group was obviously longer than the control group（P〈0.05）. Conclusion：Cationic liposome complex plasmids encoding angiostatin can inhibit the growth and metastasis of implanted Lewis lung cancer in mice model.

关 键 词：阳离子脂质体 血管抑素基因 LEWIS肺癌 小鼠移植瘤 

分 类 号：R734.2[医药卫生—肿瘤]