紫色马铃薯“黑美人”辐射处理表型及DNA变异鉴定  

Phenotypic and DNA Mutagenic Effect by Gamma Irradiation on Purple Potato(Solanum tuberosum L.)

作　　者：黄团[1] 李旭[1,2] 黄先群[1] 辛智海[2] 曹通[1,2] 李丽[1] 刘竹梓[3] 丁小令 林平 陈梦玉 

机构地区：[1]贵州省农业科学院重点实验室,贵州省生物技术研究所,贵阳550006 [2]贵州大学生命科学学院,贵阳550025 [3]贵州省亚热带作物研究所,贵阳550006 [4]贵州金农辐照科技有限责任公司,贵阳550006

出　　处：《西北农业学报》2014年第3期145-150,共6页Acta Agriculturae Boreali-occidentalis Sinica

基　　金：贵州省科技攻关项目[黔科合NY字(2011)3021];贵州省农委动植物专项[黔农育专字(2011)029];贵州省科技计划项目[黔科创能院所(2010)4006];贵州省农业科学院专项[黔农科院专项(2009)002号];贵州省农科院研究生创新基金[黔农科合(创新基金)2010012号];贵州省自然科学基金[黔科合J字(2013)2189号]

摘　　要：利用60 Coγ射线对紫色马铃薯品种黑美人萌芽薯块进行诱变处理。诱变材料表型鉴定结果表明,辐照剂量与出苗率呈显著负相关,其半致死剂量为70Gy,致死剂量为80Gy。辐照处理能显著延缓出苗时间,导致叶片减少、茎杆变细、植株变矮。高剂量处理可提高分枝数。与CK相比,各处理结薯数差异不显著。单株产量随辐照剂量增加而降低,CK处理显著高于其他处理。在表型鉴定的基础上,利用SSR分子标记对辐射材料进行DNA检测。10对引物集团选择结果表明,6对具有多态性,共获得10条多态性条带,平均每对引物有1.7条。单株检测共获得扩增条带28条,其中8条有多态性,多态性比率为28.9%。说明,辐射可引起紫色马铃薯黑美人表型和DNA水平的变异。In order to increase the mutation frequency and obtain new mutation genetic resources, budding tubers of Hemeiren,one variety of purple potato （Solanum tuberosum L. ）,were treated with ^60Coγ/irradiation at the different doses. The mutagenic effects of VM1 plants were studied by pheno- typic observation and molecular marker detection. The identified results of phenotypic variation showed that the irradiation dose was negatively correlated with the rate of seedling emergence. The half lethal dose of this variety was 70 Gy, and the lethal dose was 80 Gy. Irradiation significantly de- layed emergence time, and dwarfing plantlets, decreasing number of leaves and stem diameter. High- er doses irradiation increased number of branches. Compared with control （CK）, numbers of tubers per plant were not significantly different. The yields per plant decreased with the increase of irradia- tion dose. and the yield per plant of CK was higher than all irradiation treatments significantly. Then,DNA mutations of VM1 radiation materials were detecting by using SSR molecular marker. The re- sults showed that six polymorphic primers and ten polymorphic bands were obtained from bulk selec- tion of ten SSR primers, and the polymorphic bands per primers were 1.7 on average. Furthermore, twenty-eight amplified bands were obtained from individual detection, and eight of them were poly- morphic. With 28.9% of the polymorphism rate. In conclusion, ^60Coγ irradiation can lead to mutation of phenotype and genomic DNA on the Heimeiren variety.

关 键 词：马铃薯 ^60Coγ辐射 变异 SSR分子标记 

分 类 号：S532[农业科学—作物学]