奥曲肽对高脂饲料诱导的肥胖大鼠胰腺脂质浸润的影响  

Effects of octreotide on fatty infiltration of the pancreas in high-fat diet induced obesity rats

作　　者：余涛[1] 刘瑞[1] 李茂[1] 李献[1] 强鸥[1] 黄微[1] 唐承薇[1] 

机构地区：[1]四川大学华西医院生物治疗国家重点实验室人类疾病相关多肽研究室华西医院消化内科,成都610041

出　　处：《卫生研究》2014年第2期186-192,共7页Journal of Hygiene Research

基　　金：国家自然科学基金(No.30870919);四川省科技厅科技支撑计划(No.2010SZ0176)

摘　　要：目的探讨生长抑素类似物奥曲肽对高脂饲料诱导的肥胖大鼠胰腺脂质浸润的影响。方法雄性SD大鼠随机分为空白对照组(n=14)与高脂饲料组(n=36),分别用普通饲料及高脂饲料喂养。24周后,从高脂组大鼠中根据体重选取肥胖大鼠,肥胖大鼠再随机分为肥胖模型组(n=14)及奥曲肽干预组(n=16)。奥曲肽干预组大鼠连续8 d每12 h皮下注射奥曲肽40 mg/kg BW。实验结束后测定大鼠体重、身长,测定空腹血糖(FPG)、血胰岛素、TG、TC、HDL-C水平及胰腺组织TG和游离脂肪酸(FFA)含量,计算HOMA指数。测定胰腺组织匀浆液生长抑素(SST)水平,测定胰腺脂肪分化相关蛋白(ADFP)表达水平,观察胰腺的病理改变及脂质浸润情况。结果与空白对照组大鼠比较,肥胖组大鼠的体重、Lee’s指数、FPG、胰岛素、TG、TC水平及HOMA指数均显著升高(P<0.05),HDL-C水平则显著降低(P<0.05),胰腺组织TG及FFA含量显著升高(P<0.05),胰腺组织匀浆液SST水平显著降低(P<0.05),ADFP蛋白表达水平显著升高(P<0.05),胰腺小叶内出现明显脂肪浸润。奥曲肽干预组大鼠的体重、FPG、胰岛素、TG、TC水平及HOMA指数较肥胖组大鼠显著降低(P<0.05),胰腺组织TG及FFA含量显著降低(P<0.05),胰腺组织匀浆液SST水平显著升高(P<0.05),ADFP蛋白表达水平显著降低(P<0.05),胰腺小叶内脂肪浸润程度减轻。结论奥曲肽可通过降低血脂及血糖水平,降低胰腺ADFP的表达水平而改善高脂饲料诱导的肥胖大鼠胰腺脂质浸润和脂代谢紊乱,改善胰岛素抵抗,减少胰腺损伤。Objective To investigate effects of octreotide on fatty infiltration of the pancreas in high-fat diet induced obesity rats. Methods SD rats were divided into control group （n = 14） and high-fat diet group （n = 36）. Obese rats from the high-fat diet group were further divided into 2 groups： the obese group （n = 14） and the octreotide-treated group （n = 16）. Rats in the octreotide-treated group were subcutaneously injected with octreotide per 12 h （40 mg/kg BW） for 8 days. Body weight, fasting plasma glucose （FPG） , fasting serum insulin, triglyceride （TG） , total cholesterol （ TC ）, high-density lipoprotein cholesterol （HDL-C） levels, pancreatic TG and FFA content were measured. Homeostatic model assessment （HOMA） index was calculated. Somatostatin （SST） and the expression of adipose differentiation-related protein （ADFP） in pancrea were measured. Pathological changes of pancreas were examined with light microscopy. Results Body weight, Lee＇s index, FPG, fasting serum insulin, TG, TC levels and HOMA index in the obese group were higher than those in the control group （ P 〈 0.05）, while the level of HDL-C in the obese group was lower than that in the control group （P 〈 O. 05）. Pancreatic TG, FFA contents and expression of ADFP in the obese group were significantly higher than those in the control group （P 〈 0.05） , while pancreatic SST level in the obese group was lower than that in the control group （P 〈 0.05）. Obvious pancreatic intra- lobular fatty infiltration was observed in the obese group. After treatment of octreotide, body weight,HOMA index, as well as other plasma parameters as above showed decrease as compared with those in the obese group （P 〈 0.05）. In addition, pancreatic TG, FFA contents and the expression of ADFP in the octreotide treated group were also significantly decreased compared with those in the obese group （P 〈 O. 05 ） , pancreatic SST level was increased in the octreotide treated group than that in th

关 键 词：奥曲肽 肥胖 高脂饲料 胰腺脂肪浸润 大鼠 

分 类 号：R576[医药卫生—消化系统] Q591.5[医药卫生—内科学]