添加蛋白原料辅助固态发酵生产功能菌生物有机肥的研究  

Adding protein materials as solid-state fermentation media for producing bio-organic fertilizers with functional microbes

作　　者：曹亮亮[1] 张苗[1] 施娟娟[1] 李荣[1] 沈其荣[1] 

机构地区：[1]南京农业大学资源与环境科学学院/农业部长江中下游植物营养与肥料重点实验室/江苏省固体有机废弃物资源化高技术研究重点实验室/江苏省有机固体废弃物资源化协同创新中心,江苏南京210095

出　　处：《南京农业大学学报》2014年第2期85-91,共7页Journal of Nanjing Agricultural University

基　　金：国家自然科学基金项目(41101231);江苏省科技支撑计划(农业)项目(BE2012377);江苏省博士后科学基金项目(1102079C);国家863计划项目(2013AA102802);中国博士后科学基金项目(2011M501248;2012T50479)

摘　　要：以膨化羽毛粉和菜粕为生物有机肥添加原料,研究了其对根际促生拮抗功能菌(PGPR)固态发酵以及制成的生物有机肥对香蕉植株生长的影响及效果。结果表明:2种蛋白原料均能显著增加生物有机肥中PGPR菌株的数量,最适添加量(以干质量计)均为100 g·kg-1。添加蛋白原料制成的生物有机肥能够显著促进香蕉植株的生长,40 d时,施用添加菜粕或膨化羽毛粉制成的生物有机肥处理的地上部和地下部鲜质量分别是化肥对照的1.26倍和1.16倍与1.18倍和1.12倍,与未添加外源蛋白制成的生物有机肥处理相比,分别增加了28.7%和31.2%与20.6%和26.9%;70 d时,与施用不添加蛋白原料的生物有机肥处理相比,添加菜粕或膨化羽毛粉制成的生物有机肥处理的株高和茎粗分别增加了25.95%和10.72%与16.22%和8.74%。结论:虽然膨化羽毛粉的效果不及菜粕,但其在微生物增殖和促进植株生长方面仍具有一定优势,可以用来替代菜粕研制生物有机肥。The possibility of utilization of feather powder and rapeseed cake as solid-state fermentation media to produce bio-organic fertilizers with plant growth-promoting rhizobacteria（PGPR） strain Bacillus amyloliquefaciens NJN-6 was investigated in this study. The results showed that addition of rapeseed cake and feather powder efficiently solid-state fermented PGPR strain NJN-6 with the optimum additive amount of 100 g. kg-1（ dry weight）, improved the nutrient contents in the novel bio-organic fertilizers and significantly promote banana plant growth. After transplanting for 40 days, the fresh biomass of the above- and below-ground parts of banana plants in treatment 2 supplied with bio-organic fertilizer produced by adding rapeseed cake were 1.26 and 1.16 times of that in the CK supplied with chemical fertilizer,and increased by 28.7% and 31.2% compared to the treatment 1 supplied with bio- organic fertilizer produced with no exogenous protein resource ,while the value for banana plants in treatment 3 treated by bio-organic fertilizer produced by feather powder addition was 1.18 and 1.12 times, and increased by 20.6% and 26.9% , respectively. After transplanting for 70 days, compared to treatment 1, treatment 2 increased the plant height and stem diameter by 25.95% and 10. 72% ,respectively,while treatment 3 increased by 16.22% and 8.74% ,respectively. In conclusion, although the bio-organic fertilizer supplemented with rapeseed cake more significantly promoted banana growth than that supplemented with feather powder, the latter one still had certain advantages in solid-state fermentation of the PGPR strain and promoting banana growth, indicating a possibility to produce bio-organic fertilizer（BOF） using feather powder.

关 键 词：羽毛粉 菜粕 根际促生菌 固态发酵 生物有机肥 

分 类 号：S144[农业科学—肥料学]