虹鳟冷休克蛋白Y-box基因的cDNA全长克隆与序列分析  

The cloning and sequence analysis of Y-box gene full-length cDNA derived from Oncorhynchus mykiss

作　　者：王家庆[1] 张丽丽[2] 马爽[1] 李代宗[3] 付玉洁[1] 李绍明[1] 

机构地区：[1]沈阳工学院生命工程学院,辽宁抚顺113122 [2]沈阳农业大学科学技术学院,辽宁抚顺113122 [3]河北农业大学海洋学院,河北秦皇岛066003

出　　处：《南京农业大学学报》2014年第2期153-159,共7页Journal of Nanjing Agricultural University

基　　金：河北省科技厅科技计划项目(062424001);秦皇岛市科技计划项目(200901A068)

摘　　要：采用逆转录PCR(RT-PCR)及cDNA末端快速扩增(RACE)技术从虹鳟(Oncorhynchus mykiss)脑组织中克隆了Y-box基因cDNA全长序列(GenBank登录号:FJ492962)。该基因cDNA全长为1 452 bp,其中5'端非翻译区(5'UTR)长137 bp,3'端非翻译区(3'UTR)长409 bp,开放阅读框(ORF)长906 bp,编码301个氨基酸。蛋白质相对分子质量为33.1×103。PSORTⅡ亚细胞定位显示该蛋白位于细胞核,富含两类核定位信号,分别为pat4与pat7。虹鳟的Y-box蛋白序列与大西洋鲑、斑马鱼和大菱鲆的同源性分别为99%、80%和79%。同源建模显示虹鳟与人的Y-box蛋白具有十分相似的三级结构。系统发育树显示不同物种的Y-box蛋白分为3个亚群:亚群Ⅰ为鱼类;亚群Ⅱ为两栖类、鸟类及哺乳类;亚群Ⅲ为节肢动物类,这与物种间的进化历程基本一致。Using reverse transcription PCR （RT-PCR）and rapid amplification of cDNA ends （RACE）technique, a full-length sequence of Y-box gene of cDNA（ GenBank No. ： FJ492962 ）from rainbow trout （Oncorhynchus mykiss ）brain tissue was cloned. The full-length eDNA was 1 452 bp, with 5＇ untranslated region（ 5＇ UTR） 137 bp,3＇ untranslated region （ 3＇ UTR） 409 bp, open reading frame （ORF）906 bp, encoding 301 amino acids. The molecular weight is 33.1 × 10^3. PSORT Ⅱ subeellular localization showed that the protein was in the nucleus, which contains two kinds of nuclear localization signal of pat4 and pat7. The Y-box protein sequences homology among rainbow trout, zebrafish, Atlantic salmon and turbot were 99% , 80% and 79%. Homology modeling shows rainbow trout and human Y-box proteins had the similar tertiary structure. Phylogenetic tree showed that different species of Y-box proteins were divided into three subgroups：subgroup I for fish;subgroup Ⅱ for amphibians, birds and mammals;subgroup III for arthropod animal, which is consistent with the evolution of species

关 键 词：虹鳟 冷休克蛋白 Y-box基因 生物信息学 

分 类 号：Q38[生物学—遗传学] S917.4[农业科学—水产科学]