NZW小鼠中CD4^+T细胞活化后IL-10R1表达的研究  

The Study of the IL-10R1 Expression Level on the NZW Mouse CD4^+ T Cell after Stimulation

作　　者：刁骋[1] 曾艳[1] 杨芳莉[1] 严丹丹[1] 韦星呈[1] 

机构地区：[1]沈阳医学院基础医学院免疫学教研室,辽宁沈阳110034

出　　处：《沈阳医学院学报》2014年第1期4-7,共4页Journal of Shenyang Medical College

基　　金：辽宁省教育厅科学研究一般项目(No:L2012368);沈阳医学院科学研究基金项目(No:20121017);沈阳医学院大学生科研课题项目(No:20139029)

摘　　要：目的:检测系统性红斑狼疮小鼠(NZW小鼠)CD4+T细胞在活化后表面IL-10R1的表达变化,为进一步研究白细胞介素10(Interleukin 10,IL-10)相关免疫疾病的发病机制及进展提供有力支持。方法:取小鼠的脾细胞,经溶血后采用尼龙毛法去除B淋巴细胞,细胞经抗-CD3和抗-CD28多克隆抗体刺激,分别在活化后0,18,24和48 h采用流式细胞术以FITC抗-CD4抗体标记CD4+T淋巴细胞检测il-10r1的表达情况。结果:未活化时NZW小鼠CD4+T淋巴细胞不表达白细胞介素10受体1(interleukin receper 1,IL-10R1),在活化过程中IL-10R1开始表达,表达水平无明显波动,且表达水平远低于C57BL/6小鼠高峰时。而C57BL/6小鼠未活化时不表达IL-10R1,在活化过程中,随时间延长IL-10R1表达增加,24 h达到高峰,随后下降,48 h情况与0 h基本相同。结论:NZW小鼠体内淋巴细胞表面IL-10R1表达水平下降,IL-10对其包括调控功能在内的作用下降。并且NZW小鼠IL-10R1表达时间延长,由于IL-10R1基因免疫调控区的突变,导致IL-10对CD4+T淋巴细胞调节功能失调。这些都与系统性红斑狼疮发病及进展有关。Objective： To study the IL-10 receptor 1 （IL-10R1） expression level on the NZW mouse CIM ~ T cell and provide strong supply for the pathogenesis and progress of related autoimmune diseases. Method： The spleen cells of NZW mice were isola- ted and B cells were got rid of by nylon filter after hemolysis. CD4 ＋ T cells were isolated sterilely by flow cytometry. The IL-10R1 level was detected on the CD4 ~ T cell at 0, 12, 24 and 48 h by flow cytometry after stimulation by anti-CD3 and anti-CD28 poly- clonal antibody. Results： At 0 h, NZW mice CD4 ~ T cell did not express IL-10R1. In the process of activation IL-10R1 begin to express. The expression leveldrd not have obvious fluctuation. And the expression level was far lower than the peak of C57BL/6 mice. But on C57BL/6 mouse at 0 h CD4 ~ T cell did not express IL-10R1. In the process of activation the level of IL-10R1 were in- creased and at 24 h reached the peak. Then the level of IL-10R1 began to decrease and the level of 1L-10R1 at 48 h was similar with the level at 0 h. Conclusion： In NZW mouse the expression level of IL-10 were decreased, which causes a various of inhibition functions of IL-10 are declined. And in NZW mice IL-10R1 expression is longer. As the IL-10R1 genetic defects it causes the disor- der of IL-10 to regulate the CIM ＋ T lymphocytes. These play a role in the occurrence and development of systemic lupus erythema- tous.

关 键 词：NZW小鼠 T淋巴细胞 白细胞介素10 白细胞介素10受体 免疫调节 

分 类 号：R749.16[医药卫生—神经病学与精神病学]