TRAF5蛋白在乳腺癌中的表达  

Expression of TRAF5 Protein in Breast Carcinoma

作　　者：代文博[1] 米小轶[2] 刘楠[2] 林红[1] 王岩岩[1] 曾亮[3] 

机构地区：[1]沈阳市第一人民医院病理科,辽宁沈阳110041 [2]中国医科大学病理学教研室 [3]沈阳医学院基础医学院解剖学教研室

出　　处：《沈阳医学院学报》2014年第1期10-12,共3页Journal of Shenyang Medical College

摘　　要：目的:探讨肿瘤坏死因子受体相关因子5(tumor necrosis factor receptor-associated factor 5,TRAF5)在正常乳腺组织、乳腺癌组织及不同侵袭能力乳腺癌细胞系中的表达情况,并分析其与微血管密度的关系。方法:应用免疫组化方法分别检测TRAF5和CD34在70例乳腺癌组织和14例正常乳腺组织中的表达,并计数微血管密度。应用Western blot方法检测TRAF5在乳腺癌细胞系MDA-MB-231(高侵袭)和MCF-7(低侵袭)中的表达。结果:TRAF5表达于正常乳腺导管上皮细胞和乳腺癌肿瘤细胞胞浆内。其在正常乳腺组织、非浸润性导管癌、浸润性导管癌中的阳性表达率逐渐升高,但差异无统计学意义(P>0.05)。TRAF5在乳腺癌高侵袭细胞系中的表达量高于低侵袭细胞系,但差异无统计学意义(P>0.05)。乳腺浸润性导管癌中,TRAF5阳性表达的微血管密度明显高于阴性表达组(P<0.05)。结论:在浸润性导管癌中,TRAF5表达与微血管密度有关。Objective： To investigate the expression of tumor necrosis factor receptor-associated factor 5 （TRAF5） in normal breast, breast carcinoma tissue, and cell lines with different invasive ability and the relations between TRAF5 and microvessel density （MVD）. Methods： The expression of TRAF5 and CD34 in 70 specimens of breast carcinoma and 14 specimens of normal breast tis- sues was detected by SP immunohistochemistry separately, and MVI） was counted. The expression of TRAF5 in breast cancer cell lines, MDA-MB-231 with high invasive ability and MCF-7 with low invasive ability, was detected by Western blot. Results： TRAF5 was detected in cytoplasm of normal breast ductal epithelial ceils and breast carcinoma cells. The positive rates of TRAF5 were gradu- ally increased in normal breast tissues , in non-invasive ductal carcinoma and in invasive ductal carcinoma. But there was not signifi- cant difference （P 〉 O. 05 ）. The protein level of TRAF5 was slightly higher in MDA-MB-231 cells than that in MCF-7 cells, but there was not significant difference （P 〉 0. 05）. MVD of TRAF5 positive expression was more concentrated than negative expression in inva- sive ductal carcinoma （ P 〈 O. 05 ）. Conclusion： The expression of TRAF5 is correlated to MVD in invasive ductal carcinoma.

关 键 词：肿瘤坏死因子受体相关因子5 乳腺肿瘤 免疫组化 

分 类 号：R730.21[医药卫生—肿瘤]