细粒棘球蚴原头节mRNA测序及生物信息学初步分析  

Sequencing and bioinformatic analysis of mRNA from Echinococcus granulosus protoscolex

作　　者：朱明星[1,2,3] 王娅娜[1,2] 巨艳[4] 王志昇[3] 朱佳佳[1,2] 赵巍[1,2] 

机构地区：[1]宁夏医科大学医学科学技术研究中心,银川750004 [2]宁夏医学科学研究所,银川750004 [3]宁夏医科大学实验动物中心,银川750004 [4]宁夏疾病预防控制中心,银川750004

出　　处：《国际医学寄生虫病杂志》2014年第2期83-89,共7页International JOurnal of Medical Parasitic Diseases

基　　金：宁夏高等学校科学技术研究重点项目（NGY2010041）;Supported by the Project of Ningxia College of Science and Technology （NGY20100041）

摘　　要：目的 利用RNA-seq技术对细粒棘球蚴原头节的转录组进行测序并对测序数据进行生物信息学分析,以揭示细粒棘球蚴原头节mRNA所包含的信息. 方法 用Trizol法提取原头节总RNA,分离mRNA,利用Illumina公司的HiSeq2000高通量测序仪对mRNA序列进行测序.参考英国桑格研究院（Wellcome Trust Sanger Institute）公布的细粒棘球绦虫基因组数据,对测序数据进行拼接组装,将获得的unigene与非冗余的蛋白序列数据库（non-redundant,Nr）、全球蛋白资源数据库（universal protein,UniProt）、基因本体数据库（gene ontology,GO）以及日本京都基因和基因组百科全书通路数据库（the kyotoencyclopedia of genes and genomes pathway,KEGG pathway）进行比对,获得该unigene的蛋白功能注释信息. 结果 测序后共获得132 007 609条读段（reads）,经过组装共形成91 342条unigene,平均长度为419 bp.通过GO分类注释,共有36 552个unigene获得了注释信息,分别归入到了生物学过程、分子功能、细胞组分3大类共计48个功能组.通过KEGG pathway的注释,有6 664条unigene注释到了KEGG上,参与了6大类生物过程中34个小类的代谢,共有227个代谢通路. 结论 获得了细粒棘球蚴原头节转录组相关信息,为棘球蚴病的后续研究提供了研究数据,积累了研究资料.Objective To reveal the transcriptomic information and biological characteristics of Echinococcus granulosus protoscolex by using RNA-Seq technique and through bioinformatic analysis of sequencing data.Methods Total RNA was isolated from Echinococcus granulosus protoscolex using TRIzol.mRNA kit with Oligo（dT） magnetic beads was used to isolate poly（A） mRNA.Illumina HiSeq 2000 was applied for sequencing.Data from sequeced were assembled into unigene by using mapping-first approaches based on the genome which uploaded in the Wellcome Trust Sanger Institute.Alignment between unigenes and protein databases,such as NCBI non-redundant protein （Nr） database,UniProt database,the gene ontology （GO） database,the Kyoto Encyclopedia of Genes and Genomes （KEGG） pathway database,was performed.Results A total of 132 007 609 clean reads and 91 342 unigenes were generated.The average length of unigene was 419 bp.Through analysed with GO,26 552 unigenes were mapped.Further biological process categories of GO prominently represented in biological process,molecular functions and cellular components including 48 categories.Six thousand six hundred and sixty-four unigenes were mapped to 227 Kyoto Encyclopedia of Genes and Genomes （KEGG） pathways,including 6 first level categories and 34 second level categories.Conclusion Extensive transcriptome data from sequencing of Echinococcus granulosus protoscolex were obtained.The information has provided a database for further study on the hydatid disease.

关 键 词：细粒棘球蚴原头节 转录组 高通量转录组测序 生物信息学分析 

分 类 号：R730[医药卫生—肿瘤]