大鼠肝细胞癌变过程中survivin与livin的动态表达及相关性研究  

Dynamic expression and correlation of survivin and livin during hepatocellular carcinogenesis in rats

作　　者：李丰[1] 王炜[1] 姜开文[1] 张隆鑫 

机构地区：[1]广州医科大学附属第一医院肝胆外科,广东广州510000

出　　处：《中国医药导报》2014年第8期27-30,F0003,共5页China Medical Herald

基　　金：国家自然科学基金项目(编号30571829)

摘　　要：目的探讨大鼠肝细胞癌变过程中survivin与livin的动态表达及其二者的相互关系。方法随机将100只SD大鼠分为模型组与对照组各50只,模型组大鼠给予0.01%DEN自由饮用18周诱导大鼠肝癌模型,对照组大鼠常规自由饮水,于4、8、12、16、18周分别处死模型组10只,对照组10只。采用免疫组化、RT-PCR、Western bolt法检测survivin和livin的表达。结果免疫组化结果显示:模型组大鼠survivin、livin表达总阳性率分别为82%(41/50)、86%(43/50),survivin与livin都表达于肝癌组织及癌旁肝组织中,且二者的表达重叠,Spearman相关分析表明,二者的表达有相关性(r=0.9027,P<0.05)。RT-PCR检测结果显示,肝癌组织中均有survivin mRNA和livin mRNA表达,随着肝癌程度的加重而增加,与对照组肝组织比较,差异有统计学意义(P<0.05)。Western blot结果显示,survivin、livin的表达随着肝癌程度的加重而增加,对照组肝组织比较,差异有统计学意义(P<0.05)。结论survivin、livin在肝癌组织中高表达,且二者的表达密切相关,为肝细胞癌的临床治疗以及预后提供理论依据。Objective To determine the dynamic expression and correlation of survivin and livin during hepatocellular carcinogenesis in rats.Methods 100 SD rats were divided into model group and control group with 50 rats in each group.Rats in the model group were given 0.01%DEN for drink induced model of liver cancer,while rats in the control group were given conventional water for drink.10 rats in model group and control group were killed at 4,8,12,16,18 weeks respectively.Survivin and livin were measured by immunohistochemistry,RT-PCR,Western bolt.Results The results of immunohistochemistry showed that the total positive rates of survivin and livin were 82% （41/50）,86% （43/ 50） in the model group,survivin and livin expressed in hepatic cellular cancer tissues and para-carcinoma tissue,the expression of them was overlapping.Spearman correlation analysis showed that the correlation between them was positive （r=-0.9027,P 〈 0.05）.The results of RT-PCR showed that survivin mRNA and livin mRNA were expressed in hepatic cellular cancer tissues,increased with the liver cancer degree,compared with the control group,the difference was statistically significant （P 〈 0.05）.The results of Western blot showed that the expression of survivin and livin increased with the increase of hepatocellular carcinoma of liver tissue.There was statistically significant difference compared with the control group （P 〈 0.05）.Conclusion Survivin and livin are highly expressed in hepatic celluar Cancer tissues,and the expression of them are closely related.The results may provide a theoretical basis for the clinical treatment and prognosis of hepatocellular carcinoma.

关 键 词：大鼠 肝细胞癌 SURVIVIN LIVIN 

分 类 号：R735.7[医药卫生—肿瘤]