免疫亲和柱-高效液相色谱紫外法检测牛奶及奶粉中黄曲霉毒素M1  

Method for the determination of Aflatoxin M_1 in milk and milk powder by immunoaffinity column clean-up and HPLC-UV

作　　者：陈冬东[1] 代汉霞 彭涛[1] 姚佳[2] 王昕[2] 张建新[2] 里南 王雄 

机构地区：[1]中国检验检疫科学研究院.北京100025 [2]西北农林科技大学,陕西扬凌712100 [3]北京中检维康生物技术有限公司,北京100044

出　　处：《中国乳品工业》2014年第3期52-53,61,共3页China Dairy Industry

基　　金：国家科技支撑计划项目(2012BAK08B01)

摘　　要：建立免疫亲和柱富集净化、高效液相色谱一紫外法检测牛奶及奶粉中黄曲霉毒素M。的分析方法。该方法样品经离心处理、免疫亲和柱净化，即可应用液相色谱一紫外法检测。结果表明，不同加标水平下牛奶和奶粉添加黄曲霉毒素M，回收率分别为87．4％-108％、89．5%-103％，RSD分别为2．61％-7．83％和2．07％4．15％。方法检测灵敏度为0．1ng／mL牛奶、2．0ng／g奶粉。该方法能够特异性的检测样品中的黄曲霉毒素M，，不需荧光检测器就能达到较高的灵敏度，前处理简便，实用性强。This study developed a method for detecting aflatoxin M1 content in milk and milk powder.After purified and concentrated with immunoattinity column （IAC）, samples were determined by High performance liquid chromatography-ultraviolet detector. The recoveries of samples（milk and milk powder） with different spiking levels were 87.4%-108%,89.5%-103%. the RSD were 2.61%-7.83%, 2.07%-4.15%. The limit of quantification for milk and milk powder were 0.1 ng/mL milk and 2.0 ng/g milk powder.Tjis method can specificity detecting afla- toxin M1 content in milk and milk powder, achieving high sensitivity without fluorescence detector,was a rapid ,simple and practical method.

关 键 词：黄曲霉毒素M. 液相色谱一紫外法 免疫亲和柱 牛奶 奶粉 

分 类 号：TS252.7[轻工技术与工程—农产品加工及贮藏工程]