胃癌NK细胞浸润与预后的关系  

Relationship between intratumoral natural killer cell infiltration and prognosis in gastric carcinomas

作　　者：张声[1] 陈林莺[1] 李莉[1] 曾赛凡[1] 陈余朋[1] 王小亚 

机构地区：[1]福建医科大学附属第一医院病理科,福州350005 [2]福州迈新生物技术开发公司,福州350002

出　　处：《临床与实验病理学杂志》2000年第6期489-492,共4页Chinese Journal of Clinical and Experimental Pathology

基　　金：福建省教委科研基金!(NoJA9810 3);福建省卫生厅科研基金资助项目!(No 9710 9)资助项目

摘　　要：目的 :探讨NK细胞浸润与胃癌患者预后间的关系。方法 :应用免疫组化S P法对 15 9例有详细随访资料的胃癌组织进行CD5 7染色分析 ,通过NK细胞计数 ,将NK细胞浸润程度分为 3组 :NK细胞少量浸润组 (NK细胞浸润 <5 0个 ) ;NK细胞中量浸润组 (NK细胞数在 5 0～ 15 0个 )和NK细胞多量浸润组 (NK细胞数 >15 0个 ) ,使用Cox风险模型进行多因素分析。结果 :随着TNM分期的进展胃癌患者 5年生存率明显降低 (P <0 0 5 )。NK细胞多量浸润者 5年生存率高于NK细胞少量浸润者和中量浸润者 (P <0 0 5 ) ,同时对TNM分期后发现TNMⅢ～Ⅳ期的胃癌患者中 ,NK细胞多量浸润者的 5年生存率高于NK细胞少量浸润者和中量浸润者。多因素Cox风险模型分析发现影响胃癌患者总体生存率的因素有 :TNM分期 ,NK细胞浸润数量 ,肿瘤分化程度和肿瘤大小。结论 :胃癌患者肿瘤细胞间NK细胞多量浸润与较好的预后有关 ,并且肿瘤细胞间NK细胞浸润数量可作为判定胃癌预后的一个指标 。Purpose To study the correlation between natural killer(NK) cells tumoral infiltration and prognosis of gastric carcinomas. Methods By using immunohistochemical method,CD57 staining was performed in 159 gastric carcinomas. After the number of NK cells were counted, the degree of NK cell intratumoral infiltration was classfied into three groups: a few infiltration(<50 NK cells), moderate(50～150 NK cells) and extensive(>150 NK cells). Multivariate analysis were performed using the cox regression method. Results The 5 years survival rates was significantly decreased with the TNM stage being progressed( P <0 05). And the 5 years survival rate in the patients with NK cell extensive infiltration was higher than those in NK cells a few and moderate infiltration( P <0 05). At TNM stage Ⅲ～Ⅳ, the 5 years survival rate in patients of NK cells extensive infiltration was remarkably higher than those of a few and moderate( P <0 05). In multivariate analysis, the factors affecting overall survival rates were TNM stage, NK cells tumoral infiltration, differentiation and size of gastric carcinomas. Conclusions NK cells extensive tumoral infiltration in the gastric carcinomas is associated with favorable outcome, and the number of NK cell tumoral infiltration can be used as a variable of estimating prognosis, especially in the patients with stage Ⅲ～Ⅳ gastric carcinomas.

关 键 词：胃肿瘤 天然杀伤细胞 预后 免疫组织化学 NK细胞 肿瘤浸润 

分 类 号：R735.2[医药卫生—肿瘤] R730.23[医药卫生—临床医学]