巨痣脱细胞真皮基质残留黑色素颗粒脱除的研究  

Experimental study on removal melanin granules from acellular dermal matrix of giant nevus

作　　者：刘倩[2] 张晨[1] 刘林嶓[2] 

机构地区：[1]大连大学附属新华医院整形外科,116021 [2]郑州大学第一附属医院整形外科,450052

出　　处：《中华整形外科杂志》2014年第2期122-125,共4页Chinese Journal of Plastic Surgery

摘　　要：目的探讨一种能够避免异种和同种异体脱细胞真皮基质（acellular dermal matrix，ADM）缺点的无色素残留的自体脱细胞真皮，为自体组织回植、修复组织缺损提供实验依据。方法于32块手术切除的0．5cm×0．5cm的巨痣组织标本中，随机取其中1块以10％甲醛固定后作为巨痣细胞脱除的对照，而剩余31块巨痣组织和1块同等大小的正常皮肤组织标本则置于0．25％DispaseⅡ试剂中，室温下消化24h，去除表皮，再置于0．5％Triton X-100试剂中窒温下消化48h，之后取出正常皮肤组织和1块巨痣组织，余30块分别置于不同浓度H2O2中，于不同温度、时间进行处理，最后所有标本进行光镜、组织学等检测。结果①巨痣组织标本经0．25％DispaseII和0．5％TritonX-100脱细胞处理后，可有效地去除巨痣组织中的痣细胞及其他所有细胞成分，可见细胞脱除后留下的腔隙，未见任何细胞碎片，但仍残留部分色素。②巨痣组织标本再置于3％和10％H2O2中，分别于25、37和40℃条件下处理12～60h，可继续脱除残留黑色素颗粒。③于3％H：O：、37cc条件下处理36h，可脱除全部黑色素颗粒，Ⅰ型胶原含量无明显变化，光镜下观察胶原纤维粗细均匀，完整而连续，排列规整，无明显变性。结论经0．5％TritonX-100和0．25％DispaseⅡ脱细胞处理后的巨痣组织，进一步经3％H2O2于37℃条件下处理36h，可脱除残留黑色素颗粒，且Ⅰ型胶原蛋白含量和胶原纤维结构均无明显变化，由此制备的巨痣ADM，可考虑应用于自体组织回植、修复组织缺损。Objective To study the possibility of removal melanin granules from autogenic acellular dermal matrix of giant nevus tissue by H2O2 bleaching technique. Methods A total of 32 skin specimens （0.5 cm ×0. 5 cm） from giant nevus tissue and 1 piece（0. 5 cm×0. 5 cm） of normal skin were obtained from the surgical removal. One giant nevus tissue was chosen as control. The others and the normal skin tissue were treated with solution of 0. 25% Dispase Ⅱ for digestion for 24 hours under normal temperature to remove epidermis. Then each piece was immerged into solution of 0.5% Triton X-100 for digestion for 48 hours in normal temperature. One giant nevus tissue and the normal skin tissue were chosen as control. The others were immerged into solution of different concentrations of H202, treated under different temperature and lasting for different period. Lastly, all specimens were treated with HE staining, immunohistochemical staining, light microscopy and so on. Results After giant nevus tissues were treated with solution of 0. 25% Dispase Ⅱ and immerged into solution of 0.5% Triton X-100 in normal temperature, nevus cells and .all other cellular components of pigmented nevus tissues can be effectively removed, there were the cavities left by removal of cells without any residual cell debris, but still remaining part of pigment. Then each specimen were immerged into solution of different concentrations of H2O2, under different temperature and lasting for different period which can remove residual melanin granules. In solution of 3% H2O2for 36 h under 37℃ , can remove all the melanin particles, the content of collagen type I in the obtained specimen was not changed. Collagen fibers were uniform in thickness, regular in arrangement with no obvious degeneration. Conclusions With solution of 0.25% Dispase II and solution of 0. 5% Triton X-100 in normal temperature, all cells in nevus tissue can be removed effectively. Further treatment with 3% H202at 37 ℃ for 36 h can remove all the melanin particles, while co

关 键 词：痣 黑色素瘤 脱细胞真皮基质 过氧化氢 

分 类 号：R739.5[医药卫生—肿瘤]